Tide Fluor 2WS Amine [TF2WS amine] *Superior replacement for FITC*
Ambient (Room) temperature
Tide Fluor 2WS Amine [TF2WS amine] *Superior replacement for FITC* is a highly purified chemical compound for various applications and uses in biology, chemistry, physics, medical and other laboratories. Tide Fluor 2WS Amine [TF2WS amine] *Superior replacement for FITC* is available in bulk quantities in which case a discounted pricing will be applied in accordance with the amount. Please, note that Tide Fluor 2WS Amine [TF2WS amine] *Superior replacement for FITC* is intended for research and development purposes and should not be used as a medication.
Whether the chemical is in the form or in liquid, small amounts may get caught on the vial's walls and/or lid due to shipping and handling factors. We recommend you to briefly centrifuge the vial prior to useage to ensure that all of the chemical is on the bottom of the vial.
This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
Scope note:Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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