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Available ordering format
Lyophilized, for reconstitution add 1 ml of sterile water
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Immunogen
Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b.
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Raised in
Goat
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Purified IgG
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Stored lyophilize material at +4°C; and for long time storage at -20°C. After reconstirution make aliquots and store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thawed the aliquotslowly in the dark at ambient temperature, spun down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day.
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Verified applications
ELISA (ELISA), Immunocytochemistry (ICH), Immunohistochemistry (IHC)(frozen), immunofluorescence (IF)
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Connected products
other FITC conjugated secondary antibodies to mouse IgG
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Recommended dilutions for use
1:20 to 1:80The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
Mouse IgG2a and IgG2b, subclass specific
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Possible reactivity
to be determined
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No reactivity
It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
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Supplementary information
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References
not available, antibody released in November 2015.
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Scientific context
Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugated is a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.
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Notes
If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.The reactivity of this product is directed to the subclasses IgG2a and IgG2b as tested against mouse sera other purified homogenous immunoglobulins.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
This agisera Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. agisera FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
