FITC Conjugated Avidin

• Catalog number
  BA1125-0.5
• Price
  Please ask
• Size
  500 µl

• Type of the antibody protein
  Avidin
• Raised in synthesized
  extracted avidin
• Conjugation
  FITC
• Product configuration
  Solution with concentration 1mg/ml
• Immunogen
  N/A
• Clonality
  Avidin
• Analyses
  IHC-P,IHC-F,ICC,FCM
• Storage conditions
  Keep the FITC Conjugated Avidin at plus four degrees Celsius for 12 months.
• Product details
  Avidin is a 68KD protein that extracted from egg white. It has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is an alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction.
• Tips
  The FITC Conjugated Avidin is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
• Properties
  This boster Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. boster FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody

• Gene target
  Conjugated   Avidin  
• Short name
  FITC Conjugated Avidin
• Technique
  FITC, antibody Conjugates, Fluorescein
• Label
  FITC
• Alternative name
  fluorecein coupled Avidin
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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