HtrA4 maxi Extraction & Western Blot Kit
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Catalog number
30 501 404
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Price
Please ask
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Size
10 extraction & 10 blots
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ELISA category
Extraction & Western Blot Kit
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ELISA detection limits
0.05 - 2.5 µg/extract
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ELISA sensitivity
40-50 ng/µl Beads (bindungs-capacity)
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ELISA s description
This package provides the EXTRACT maxi HtrA4 kit together with a high specific monoclonal antibody against human HtrA4 full length and a recombinant HtrA4 protein control for Western Blot. Each kit provides sufficient reagents for preparing 10 extracts for an enrichment of HtrA4 about 2-2.4 µg per extract and for 10 detection of human HtrA4 with Western Blot.
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Research area interests
Cancer, Rheumatic Diseases, Arthritis, Cardiovascular, Cell Biology, Signal Transduction, Immunology
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Package form
single tube
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Antigen
HtrA4
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Reacts with
HtrA4
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Tested applications
This package provides the EXTRACT maxi HtrA4 kit together with a high specific monoclonal antibody against human HtrA4 full length and a recombinant HtrA4 protein control for Western Blot. Each kit provides sufficient reagents for preparing 10 extracts for an enrichment of HtrA4 and for 10 detection of human HtrA4 with Western Blot.
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Specificity
HtrA4
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Crossreactivity
N/A
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Preservative materials
If applicable mentioned in the product data sheet
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Storage recommendations
6 month
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Shipping and storage conditions
ice pack
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Long term storage
-20°C and 2-8°C
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Assay principle
specific Affinity chromatography and Western Blot
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Included in the standard package
buffers, antibodys antibody conjugate,standards, control
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Not included in the standard package
Spectrophotometer, Pipettes or pipetting equipment, Disposable polypropylene test tubes, Distilled or deionised water
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Samples to be analyzed
biological fluids, tissues, cell culture
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PubMed citations
See the datasheet
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Technical datasheet
Contact us to receive the datasheet
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Representative figures
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Warnings
For Research Use only.
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Test
Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
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Gene target
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Short name
HtrA4 maxi Extraction & Blot Kit
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Technique
Western Blot, Blot, western Blots
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Alternative name
HtrA4 maxi Extraction & Western Blot reagent
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Alternative technique
western, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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