Sphingosine Kinase 2 (N Terminal HIS Tag) - Active Enzyme

• Catalog number
  X1709E
• Price
  Please ask
• Size
  10 μg

• Category
  Proteins & Peptides
• Long description
  Sphingosine Kinase 2 (Sphk2) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions. Inside the cell S1P acts as a signaling molecule like other sphingolipid metabolites like ceramide and sphingosine. S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis. The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors). These receptors couple to multiple G proteins (e.g. S1P1 couples to Gi whereas S1P2 and S1P3 couple to Gq, G13 in addition to Gi) and regulate a extremely wide raange of cellular events including cell motility, survival, apoptosis, migration and cell-cell interaction. Important roles for S1P have also been reported in regulation of cardiogenesis, vascular maturation, oocyte survival, immune cell trafficking, cells of the neuronal system and bone cells. S1P levels are regulated by the activity of Sphk (Sphk1 and Sphk2).
• Antibody come from
  Hybridoma produced by the fusion of splenocytes from mice immunized with a keratin isolated from the human breast carcinoma cell line MCF-7 and mouse myeloma cells.
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide.
• Clone
  not specified
• Antigen antibody binding interaction
  Sphingosine Kinase 2 (N Terminal HIS Tag) - Active Enzyme
• Antibody is raised in
  Sf9 cells
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Antibody s suited for
  Antibody can be used for immunoblotting (1:100-1:1000), immunocytochemistry, immunohistochemistry on frozen tissue sections (1:100-1:200) and flow cytometry. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -80ºC
• Relevant references
  1. Van Muijen, G. N., et al. (1987). Coexpression of intermediate filament polypeptides in human fetal and adult tissues, Lab Invest 57, 359-69._x000B_2. Van Muijen, G. N., et al. (1987). Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45._x000B_3. Schaafsma, H. E., et al. (1989). Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract, Histochemistry 91, 151-9._x000B_4. Smedts, F., et al. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68._x000B_5. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B_6. Schaafsma, H. E., et al. (1990). Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43._x000B_7. Ivanyi, D., et al. (1990). Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis, Cancer Res 50, 5143-52._x000B_8. Wetzels, R. H., et al. (1991). Basal cell-specific and hyperproliferation-related keratins in human breast cancer, Am J Pathol 138, 751-63._x000B_9. Ku, N. O., et al. (2001). Keratin 8 mutations in patients with cryptogenic liver disease, N Engl J Med 344, 1580-7._x000B_10. Waseem, A., Karsten, U., Leigh, I.M., Purkis, P., Waseem, H. and Lane, B. (2004). Conformational changes in the rod domain of human keratin 8 followingheterotypic association with keratin 18 and its implication for filament stability. Biochemistry 43, 1283-95.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
• Conjugation
  histidine

• Gene target
  Sphingosine   Kinase   Terminal   HIS   Tag   Active  
• Gene symbol
  HAL, VIS1, AKR1B10, TRH-GTG1-1, TRH-GTG1-3, TRH-GTG2-1, TRH-GTG3-1, TRH-GTG1-2, TRH-GTG1-4, TRH-GTG1-5
• Short name
  Sphingosine Kinase 2 (N Terminal HIS Tag) - Active Enzyme
• Technique
  Enzyme, enzymes, Tags
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Sphingosine phosphorylation catalyst 2 (N Terminal histidine detection labelled) - functionnal Enzyme
• Alternative technique
  enzymes

Gene info

• Identity
  HGNC:4806
• Gene
  HAL
• Long gene name
  histidine ammonia-lyase
• Synonyms gene
  • HIS
• Locus
  12q23.1
• Discovery year
  1988-08-31
• Entrez gene record
  3034
• VEGA ID
  OTTHUMG00000170354

Gene info

• Identity
  HGNC:12696
• Gene
  VIS1
• Long gene name
  viral integration site 1
• Synonyms
  • HIS1
  • HIS-1
• GenBank acession
  • U56440
• Locus
  2q14-q21
• Discovery year
  1992-11-25
• Entrez gene record
  7435
• Pubmed identfication
  • 1682866

Gene info

• Identity
  HGNC:382
• Gene
  AKR1B10
• Long gene name
  aldo-keto reductase family 1 member B10
• Synonyms gene
  • AKR1B11
• Synonyms gene name
  • aldo-keto reductase family 1, member B10 (aldose reductase)
• Synonyms
  • AKR1B12
  • ARL-1
  • HIS
  • ARL1
  • HSI
  • ALDRLn
• Synonyms name
  • aldose reductase-like 1
  • aldo-keto reductase family 1, member B11 (aldose reductase-like)
  • aldose reductase-like peptide
  • aldose reductase-related protein
  • small intestine reductase
• GenBank acession
  • AF052577
• Locus
  7q33
• Discovery year
  2000-08-24
• Entrez gene record
  57016
• Pubmed identfication
  • 9765596
  • 9565553
• RefSeq identity
  • NM_020299
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000155356

Gene info

• Identity
  HGNC:34535
• Gene
  TRH-GTG1-1
• Long gene name
  tRNA-His (anticodon GTG) 1-1
• Synonyms gene
  • TRNAH4
• Synonyms gene name
  • transfer RNA histidine 4 (anticodon GUG)
  • transfer RNA-His (GTG) 1-1
• Synonyms
  • tRNA-His-GTG-1-1
• GenBank acession
  • HG983832
• Locus
  1q21.1
• Discovery year
  2008-08-21
• Entrez gene record
  100188991
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34874
• Gene
  TRH-GTG1-3
• Long gene name
  tRNA-His (anticodon GTG) 1-3
• Synonyms gene
  • TRNAH9
• Synonyms gene name
  • transfer RNA histidine 9 (anticodon GUG)
  • transfer RNA-His (GTG) 1-3
• Synonyms
  • tRNA-His-GTG-1-3
• GenBank acession
  • HG983834
• Locus
  1q21.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189307
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34918
• Gene
  TRH-GTG2-1
• Long gene name
  tRNA-His (GTG) 2-1
• Synonyms gene
  • TRNAH11
• Synonyms gene name
  • transfer RNA histidine 11 (anticodon GUG)
  • transfer RNA-His (GTG) 2-1
• Synonyms
  • tRNA-His-GTG-2-1
• GenBank acession
  • HG983841
• Locus
  1
• Discovery year
  2008-08-29
• Entrez gene record
  100189350
• Classification
  • Low confidence cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34805
• Gene
  TRH-GTG3-1
• Long gene name
  tRNA-His (anticodon GTG) 3-1
• Synonyms gene
  • TRNAH8
• Synonyms gene name
  • transfer RNA histidine 8 (anticodon GUG)
  • transfer RNA-His (GTG) 3-1
• Synonyms
  • tRNA-His-GTG-3-1
• GenBank acession
  • HG983842
• Locus
  3q24
• Discovery year
  2008-08-29
• Entrez gene record
  100189242

Gene info

• Identity
  HGNC:34902
• Gene
  TRH-GTG1-2
• Long gene name
  tRNA-His (anticodon GTG) 1-2
• Synonyms gene
  • TRNAH10
• Synonyms gene name
  • transfer RNA histidine 10 (anticodon GUG)
  • transfer RNA-His (GTG) 1-2
• Synonyms
  • tRNA-His-GTG-1-2
• GenBank acession
  • HG983833
• Locus
  1q21.1
• Discovery year
  2008-08-29
• Entrez gene record
  100189334
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34736
• Gene
  TRH-GTG1-4
• Long gene name
  tRNA-His (anticodon GTG) 1-4
• Synonyms gene
  • TRNAH7
• Synonyms gene name
  • transfer RNA histidine 7 (anticodon GUG)
  • transfer RNA-His (GTG) 1-4
• Synonyms
  • tRNA-His-GTG-1-4
• GenBank acession
  • HG983835
• Locus
  1q21.2
• Discovery year
  2008-08-29
• Entrez gene record
  100189174
• Classification
  • Cytoplasmic transfer RNAs

Gene info

• Identity
  HGNC:34538
• Gene
  TRH-GTG1-5
• Long gene name
  tRNA-His (anticodon GTG) 1-5
• Synonyms gene
  • TRNAH3
  • TRH-GTG1-8
• Synonyms gene name
  • transfer RNA histidine 3 (anticodon GUG)
  • transfer RNA-His (GTG) 1-8
  • transfer RNA-His (GTG) 1-5
• Synonyms
  • tRNA-His-GTG-1-5
• GenBank acession
  • HG983839
• Locus
  6p22.1
• Discovery year
  2008-09-03
• Entrez gene record
  100188987
• Classification
  • Cytoplasmic transfer RNAs

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