Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme)

• Catalog number
  X1106P
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Proinflammatory cytokine tumor necrosis factor- (TNF- ) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF- , notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF- , notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).
• Antibody come from
  Synthetic peptide corresponding to amino acids 732 to 748 off the human ADAM10. Immungen sequence is identical in bovine and rat and differs from the mouse protein by one amino acid.
• Other description
  Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme) Antibody
• Antibody is raised in
  Rabbit
• Antibody s reacts with
  Human, Mouse, Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot
• Antibody s suited for
  Detects ADAM10 by Western blot at 0.25 to 1 µg/ml. Detects an 85 kDa band can be detected in Jurkat cell lysates which may represent precursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat, which may be processed mature protein. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Rosendahl, M.S., et al., Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases. J. Biol. Chem. 1997, 272, 24588-24593_x000B__x000B_2. Pan, D. & Rubin, G.M., Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997, 90, 271-280_x000B__x000B_3. Qi, H., et al., Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999, 283, 91-94 _x000B__x000B_4. Howard, L., et al., Molecular cloning of MADM: a catalytically active mammalian disintegrin-metalloprotease expressed in various cell types. Biochem. J. 1996, 317, 45-50
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
• About
  Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
• Latin name
  Oryctolagus cuniculus

• Gene target
  ADAM   kuz   TNFa   converting  
• Gene symbol
  ADAMTS10, ADAMTS6, ADAMTS8, ADAMTS7, ADAMTS2
• Short name
  Rabbit anti ADAM 10 (CT) (kuz) (TNFa converting enzyme)
• Technique
  Enzyme, Rabbit, anti, antibody to, enzymes
• Host
  Rabbit, Rabbits
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  production species: rabbit antibody to ADAM 10 (CT) (kuz) (TNFa converting enzyme)
• Alternative technique
  enzymes, rabbit-anti, antibodies

Gene info

• Identity
  HGNC:13201
• Gene
  ADAMTS10
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 10
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10
• Synonyms
  • ADAM-TS10
• GenBank acession
  • AF163762
• Locus
  19p13.2
• Discovery year
  2001-04-05
• Entrez gene record
  81794
• Pubmed identfication
  • 15355968
  • 15368195
• RefSeq identity
  • NM_030957
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000182216

Gene info

• Identity
  HGNC:222
• Gene
  ADAMTS6
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 6
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 6
• Synonyms
  • ADAM-TS6
• Synonyms name
  • a disintegrin and metalloproteinase with thrombospondin motifs 6
• GenBank acession
  • AF140674
• Locus
  5q12.3
• Discovery year
  1999-09-06
• Entrez gene record
  11174
• Pubmed identfication
  • 10464288
• RefSeq identity
  • NM_197941
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000074079

Gene info

• Identity
  HGNC:224
• Gene
  ADAMTS8
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 8
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 8
• Synonyms
  • METH2
  • FLJ41712
  • ADAM-TS8
• GenBank acession
  • AF060153
• Locus
  11q24.3
• Discovery year
  1999-08-23
• Entrez gene record
  11095
• Pubmed identfication
  • 10438512
• RefSeq identity
  • NM_007037
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000165656

Gene info

• Identity
  HGNC:223
• Gene
  ADAMTS7
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 7
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 7
• Synonyms
  • ADAM-TS7
  • DKFZp434H204
• Synonyms name
  • COMPase
  • a disintegrin and metalloprotease with thrombospondin motifs-7 preproprotein
• GenBank acession
  • AF140675
• Locus
  15q25.1
• Discovery year
  1999-09-06
• Entrez gene record
  11173
• Pubmed identfication
  • 10464288
• RefSeq identity
  • NM_014272
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000172907

Gene info

• Identity
  HGNC:218
• Gene
  ADAMTS2
• Long gene name
  ADAM metallopeptidase with thrombospondin type 1 motif 2
• Synonyms gene name
  • a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2
• Synonyms
  • ADAMTS-3
  • hPCPNI
  • PCINP
  • ADAM-TS2
  • NPI
• Synonyms name
  • procollagen I N-proteinase
  • procollagen N-endopeptidase
• GenBank acession
  • AJ003125
• Locus
  5q35.3
• Discovery year
  1999-04-15
• Entrez gene record
  9509
• Pubmed identfication
  • 10094461
  • 15373769
• RefSeq identity
  • NM_014244
• Classification
  • ADAM metallopeptidases with thrombospondin type 1 motif
• VEGA ID
  OTTHUMG00000130915

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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