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TNF alpha (mouse) ELISA Kit

  • Catalog number
    K1051-100
  • Product name
    TNF alpha (mouse) ELISA Kit
  • Size
    100 assays
  • Price
    703.00 EUR
  • Supplier
  • Kit's description
    BioVision’s mouse TNF alpha ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for mouse TNF alpha coated on a 96-well plate. Standards and test samples are added to the wells and TNF alpha present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for TNF alpha is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse TNF alpha captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml.
  • Highlights
    • Detection method: Absorbance (450 nm) • Species reactivity: mouse • Application: Quantitative protein detection, establishing normal range etc.
  • Kit's benefits
    • Easy, convenient and time-saving method to assay for mouse TNF alpha. • Detection Range:15.6 pg/ml – 1000 pg/ml (cell culture supernates), 7.8 pg/ml – 500 pg/ml (mouse serum, plasma). Sensitivity: < 1 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
  • Category
    TNF alpha
  • Kit's other name
    Tumor Necrosis Factor alpha (mouse) ELISA Kit
  • Contents
    • TNF-α mAb coated plate, 96 wells • Mouse TNF-α standard (10 ng/vial) • Biotinylated anti-mouse TNF-α Ab • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB (Colorless) • TMB stop solution
  • Storage condition
    -20°C
  • Shipping condition
    gel pack
  • Maximum time can be stored
    12 months
  • Species reactivity
    mouse
  • Applications
    Application- Quantitative protein detection, establishing normal range.
  • Samples tested
    • Serum & plasma (heparin, EDTA) • Cell culture supernatants
  • Description
    The TNF alpha (mouse) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene
    Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
  • Gene target
  • Short name
    TNF alpha (mouse) ELISA Kit
  • Technique
    ELISA kit, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Mouse, Mouses
  • Alternative name
    tumor necrosis factor a (mouse) Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, this GO :0000060 and protein import into nucleus, translocation and biological process this GO :0000122 and negative regulation of transcription from RNA polymerase II promoter and biological process this GO :0000165 and MAPK cascade and biological process this GO :0000185 and activation of MAPKKK activity and biological process this GO :0000187 and activation of MAPK activity and biological process this GO :0001666 and response to hypoxia and biological process this GO :0001775 and cell activation and biological process this GO :0001819 and positive regulation of cytokine production and biological process this GO :0001891 and pha this GO cytic cup and cellular component this GO :0001932 and regulation of protein phosphorylation and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0002020 and protease binding and molecular function this GO :0002037 and negative regulation of L-glutamate transport and biological process this GO :0002439 and chronic inflammatory response to antigenic stimulus and biological process this GO :0002740 and negative regulation of cytokine secretion involved in immune response and biological process this GO :0002876 and positive regulation of chronic inflammatory response to antigenic stimulus and biological process this GO :0002925 and positive regulation of humoral immune response mediated by circulating immunoglobulin and biological process this GO :0003009 and skeletal muscle contraction and biological process this GO :0005125 and cytokine activity and molecular function this GO :0005164 and tumor necrosis factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005622 and intracellular and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006006 and glucose metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006919 and activation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0006927 and transformed cell apoptotic process and biological process this GO :0006952 and defense response and biological process this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0006959 and humoral immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0007254 and JNK cascade and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008285 and negative regulation of cell proliferation and biological process this GO :0008625 and extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0009314 and response to radiation and biological process this GO :0009612 and response to mechanical stimulus and biological process this GO :0009615 and response to virus and biological process this GO :0009651 and response to salt stress and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0010693 and negative regulation of alkaline phosphatase activity and biological process this GO :0010888 and negative regulation of lipid storage and biological process this GO :0014823 and response to activity and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0019722 and calcium-mediated signaling and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0030730 and sequestering of triglyceride and biological process this GO :0031334 and positive regulation of protein complex assembly and biological process this GO :0031622 and positive regulation of fever generation and biological process this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032715 and negative regulation of interleukin-6 production and biological process this GO :0032722 and positive regulation of chemokine production and biological process this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032741 and positive regulation of interleukin-18 production and biological process this GO :0032755 and positive regulation of interleukin-6 production and biological process this GO :0032800 and receptor biosynthetic process and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0033209 and tumor necrosis factor-mediated signaling pathway and biological process this GO :0034116 and positive regulation of heterotypic cell-cell adhesion and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0042493 and response to drug and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0042802 and identical protein binding and molecular function this GO :0043065 and positive regulation of apoptotic process and biological process this GO :0043068 and positive regulation of programmed cell death and biological process this GO :0043122 and regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0043242 and negative regulation of protein complex disassembly and biological process this GO :0043243 and positive regulation of protein complex disassembly and biological process this GO :0043280 and positive regulation of cysteine-type endopeptidase activity involved in apoptotic process and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0043507 and positive regulation of JUN kinase activity and biological process this GO :0043525 and positive regulation of neuron apoptotic process and biological process this GO :0044130 and negative regulation of growth of symbiont in host and biological process this GO :0044212 and transcription regulatory region DNA binding and molecular function this GO :0045071 and negative regulation of viral genome replication and biological process this GO :0045080 and positive regulation of chemokine biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045123 and cellular extravasation and biological process this GO :0045416 and positive regulation of interleukin-8 biosynthetic process and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045599 and negative regulation of fat cell differentiation and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045670 and regulation of osteoclast differentiation and biological process this GO :0045672 and positive regulation of osteoclast differentiation and biological process this GO :0045760 and positive regulation of action potential and biological process this GO :0045840 and positive regulation of mitosis and biological process this GO :0045892 and negative regulation of transcription, DNA-templated and biological process this GO :0045893 and positive regulation of transcription, DNA-templated and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0045994 and positive regulation of translational initiation by iron and biological process this GO :0046325 and negative regulation of glucose import and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0048566 and embryonic digestive tract development and biological process this GO :0048661 and positive regulation of smooth muscle cell proliferation and biological process this GO :0050708 and regulation of protein secretion and biological process this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050796 and regulation of insulin secretion and biological process this GO :0050806 and positive regulation of synaptic transmission and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0050900 and leukocyte migration and biological process this GO :0050901 and leukocyte tethering or rolling and biological process this GO :0050966 and detection of mechanical stimulus involved in sensory perception of pain and biological process this GO :0050995 and negative regulation of lipid catabolic process and biological process this GO :0051023 and regulation of immunoglobulin secretion and biological process this GO :0051044 and positive regulation of membrane protein ectodomain proteolysis and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051222 and positive regulation of protein transport and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051533 and positive regulation of NFAT protein import into nucleus and biological process this GO :0051798 and positive regulation of hair follicle development and biological process this GO :0051897 and positive regulation of protein kinase B signaling and biological process this GO :0055037 and recycling endosome and cellular component this GO :0060557 and positive regulation of vitamin D biosynthetic process and biological process this GO :0060559 and positive regulation of calcidiol 1-monooxygenase activity and biological process this GO :0060664 and epithelial cell proliferation involved in salivary gland morphogenesis and biological process this GO :0060693 and regulation of branching involved in salivary gland morphogenesis and biological process this GO :0061048 and negative regulation of branching involved in lung morphogenesis and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071230 and cellular response to amino acid stimulus and biological process this GO :0071316 and cellular response to nicotine and biological process this GO :0071407 and cellular response to organic cyclic compound and biological process this GO :0071677 and positive regulation of mononuclear cell migration and biological process this GO :0071803 and positive regulation of podosome assembly and biological process this GO :0097190 and apoptotic signaling pathway and biological process this GO :0097191 and extrinsic apoptotic signaling pathway and biological process this GO :0097527 and necroptotic signaling pathway and biological process this GO :1990268 and response to this GO ld nanoparticle and biological process this GO :2000010 and positive regulation of protein localization to cell surface and biological process this GO :2000343 and positive regulation of chemokine (C-X-C motif) ligand 2 production and biological process this GO :2000377 and regulation of reactive oxygen species metabolic process and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, transcription regulatory region DNA binding, this GO :0002020: protease binding and also this GO :0005125: cytokine activity and also this GO :0005164: tumor necrosis factor receptor binding and also this GO :0005515: protein binding and also this GO :0042802: identical protein binding and also this GO :0044212: transcription regulatory region DNA binding, this GO :0002020: protease binding, this GO :0005125: cytokine activity, this GO :0005164: tumor necrosis factor receptor binding, this GO :0005515: protein binding, this GO :0042802: identical protein binding, this GO :0044212: transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
MESH Data
  • Name
  • Concept
    Scope note:An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    adverse effects, classification, economics, history, instrumentation, methods, mortality, nursing, psychology, standards, trends, utilization, veterinary, statistics & numerical data, contraindications, ethics
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