Min length of search pharse is two chars

Filters
US: (408) 780-0908 | EU: 0032 16 58 90 45 lieven@gentaur.com

Mouse BMP-4 PicoKine ELISA Kit

  • Catalog number
    EK0316
  • Product name
    Mouse BMP-4 PicoKine ELISA Kit
  • Size
    1x96 well plate
  • Price
    596EUR
  • Supplier
    boster
  • Ask
  • ELISA's application
    For quantitative measurement of mouse BMP-4 in bone tissue, cell culture supernatant and serum.
  • Gene tested
    murine BMP-4
  • ELISA standard curve
    Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.045 + 0.06 + 0.094 + 0.165 + 0.333 + 0.706 + 1.437 + 2.283
  • ELISA kit detection limits
    31.2pg/ml to 2000pg/ml
  • ELISA's sensitivity
    < 10pg/ml
  • Target of this ELISA
    Natural and recombinant mouse BMP-4
  • Cross reaction
    the ELISA does not cross react with any other proteins except from closely related species
  • ELISA's precision parameters
    intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);160;556;1526;178;603;1789 ELISA standard deviations 5.12;22.80;85.46;9.43;41.00;132.39 CV(%);3.2;4.1;5.6;5.3;6.8;7.4
  • ELISA kit description
    GENTAUR's mouse BMP-4 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for BMP-4 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S293-R408) and your samples are measured with a biotin conjugated polyclonal from goat specific for BMP-4 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse BMP-4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
  • Detector and capure antibodies
    A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
  • The ELISA contains
    Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse BMP-4 antibodies;1 ST0000-10;Lyophilized recombinant mouse BMP-4 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse BMP-4 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
  • Anti-coagulation
    /
  • How the elisa works
    Sample the amount of 100ul/well of the standard solution at 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BMP-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse bone tissue, cell culture supernatant or serum to the ELISA plate. Keep in mind that each mouse BMP-4 standard solution and each sample be measured in duplicate.
  • Minutes of TMB incubation
    25-30
  • More gene info
    Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.
  • Antigen
    CHO, S293-R408
  • Keep at
    Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
  • Uniprot
    Bone morphogenetic protein 4
  • Gene name
    bone morphogenetic protein 4
  • Alternative
    BMP 2B;BMP 4;BMP2B;BMP2B1;BOMPR4A;Bone morphogenetic protein 2B;Bone morphogenetic protein 4;DVR4;OTTMUSP00000021065; OTTMUSP00000021066;ZYME
  • Gen protein identity
    P21275
  • Entrez nr.
    12159
  • Properties
    E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    BMP4
  • Short name
    Mouse BMP-4 PicoKine ELISA Kit
  • Technique
    ELISA kit, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
  • Host
    mouse, antigen assay ELISA kit 1 Kit (96 Wells)
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse BMP-4 PicoKine Enzyme-linked immunosorbent assay reagent
  • Alternative technique
    elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Ask
1. Gene info
MESH Data
  • Name
  • Concept
    Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
  • Tree numbers
    • E05.478.566.350.170
    • E05.478.566.380.360
    • E05.478.583.400.170
    • E05.601.470.350.170
    • E05.601.470.380.360
  • Qualifiers
    adverse effects, classification, economics, history, instrumentation, methods, mortality, nursing, psychology, standards, trends, utilization, veterinary, statistics & numerical data, contraindications, ethics
Related products
Chat with gentaur.com employee