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Cyclic GMP EIA Kit

  • Catalog number
    SKT-211-96
  • Product name
    Cyclic GMP EIA Kit
  • Size
    96 well
  • Price
    575.02
  • Supplier
    StressMark kits
  • Stock availability
    In Stock
  • Kit's description
    Quantitative colorimetric detection of cGMP
  • Protein target
    Cyclic GMP
  • Scientific context
    Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase on GTP and degraded through phosphodiesterase hydrolysis (1-3). Guanylate cyclases (GC) are either soluble or membrane bound (3,4). Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels (5,6). Cyclic GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters, and through its hydrolytic degradation by phosphodiesterase (1,7)
  • Primary research area
    Cell Signaling, Neuroscience
  • Category
    Assay EIA Kits
  • Other name
    cGMP; 3',5'-cyclic GMP EIA Kit, Guanosine cyclic monophosphate EIA Kit, Cyclic 3',5'-GMP; Guanosine 3',5'-cyclic phosphate EIA Kit
  • Brand name
    StressXpress®
  • Detection system
    Colorimetric Assay
  • Assay format
    Direct EIA (Enzyme Immunoassay)
  • Main intended usage
    EIA kit used to quantitatively measure cGMP present in samples.
  • Brief protocol
    The Cyclic GMP (cGMP) EIA kit is designed to quantitatively measure cGMP present in lysed cells, EDTA plasma, urine, saliva and tissue culture media samples. For samples where the levels of cGMP are expected to be relatively high, the regular format for the assay can be used. For samples with expected low levels of cGMP, an optional acetylation protocol can be used. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, which contains special additives and stabilizers, for cGMP measurement. This allows plasma, urine and saliva samples to be read in an identical manner to lysed cells. Acidified samples of cGMP are stable and endogenous phosphodiesterases are inactivated in the Sample Diluent. A cGMP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. A clear microtiter plate coated with an antibody to capture mouse IgG is provided and a neutralizing Plate Primer solution is added to all the used wells. Standards or diluted samples, either with or without acetylation, are pipetted into the primed wells. A cGMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a mouse monoclonal antibody to cGMP to each well. After a 2 hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound cGMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cGMP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
  • Kit contents
    Coated Clear 96 Well Plates, Cyclic GMP Standard, StressXpress® Cyclic GMP Antibody, StressXpress® Cyclic GMP Conjugate, Sample Diluent, Plate Primer, Acetic Anhydride, Triethylamine, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
  • Assay precision
    Intra Assay Precision (Regular): Three human urine samples were diluted with Sample Diluent and run in replicates of 20 in an assay. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.5 pmol/mL, 6.3% CV Sample 2- 4.0 pmol/mL, 7.8% CV Sample 3- 1.2 pmol/mL, 13.6% CV Intra Assay Precision (Acetylated): Three human urine samples were diluted with Sample Diluent, acetylated and run in replicates of 20 in an assay. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.1 pmol/mL, 3.8% CV Sample 2- 3.8 pmol/mL, 5.2% CV Sample 3- 1.2 pmol/mL, 8.2% CV Inter Assay Precision (Regular): Three human urine samples were diluted with Sample Diluent and run in duplicates in twelve assays run over multiple days by three operators. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.5 pmol/mL, 6.5% CV Sample 2- 4.3 pmol/mL, 7.0% CV Sample 3- 1.5pmol/mL, 8.3% CV Inter Assay Precision (Acetylated): Three human urine sample weres diluted with Sample Diluent, acetylated and run in duplicates in twelve assays run over multiple days by three operators. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.7 pmol/mL, 12.3% CV Sample 2- 4.3 pmol/mL, 14.3% CV Sample 3- 1.5 pmol/mL, 14.2% CV
  • Assay cross-reactivity
    Species Independent
  • Samples to be used with this kit
    Cell Lysates, Tissue, Saliva, Urine, EDTA Plasma, Tissue Culture Media
  • Maximum samples to be used with this kit
    39 samples in duplicate
  • Assay duration
    See product datasheet or feel free to contact us
  • Kit's sensitivity
    0,28 pmol/ml
  • Assay detection limit
    Regular: 0,5 - 32 pmol/ml, Acetylated: 0,3125 - 20 pmol/ml
  • Storage recommendations
    4°C
  • Shipping recommendations
    Blue Ice
  • Bibliography
    1. Domek-Lopacinska, K. and Strosznajder, JB. (2005) J Physiol Pharmacol 56 Suppl 2, 15-34. 2. Lucas, K.A. et al. (2000) Pharmacol Rev 52: 375-414. 3. Ashman, DF., et al. (1963) , Biochem Biophys Res Comm, 11: 330-4. 4. Potter LR, Abbey-Hosch S, and Dickey DM. (2006) Endocr Rev 27: 47-72. 5. Waldman, SA and Murad, F. (1987) Pharmacol Revs, 39: 163-197. 6. Tremblay J, Gerzer R, and Hamet P. (1988) , Adv. 2nd Messanger & Phosphoprotein Res., 22: 319-383. 7. Matsumoto T, Kobayashi T, and Kamata K. (2003) J Smooth Muscle Res 39: 67-86.
  • Release date
    17-Sep-2014
  • PubMed number
    Refer to PubMed
  • Tested applications
    No data available / currently testing
  • Tested reactivity
    No data available / currently testing
  • Resources available upon request
    Kit Booklet, MSDS, Saliva Sample Handling Instructions
  • Product image link
  • Product image legend
    Regular Format Typical Standard Curve for the Cyclic GMP EIA Kit (Enzyme Immunoassay) StressXpress® - SKT-211. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.5 - 32 pmol/ml. Acetylated: 0.3125 - 20 pmol/ml. | Acetylated Format Typical Standard Curve for the Cyclic GMP EIA Kit (Enzyme Immunoassay) StressXpress® - SKT-211. Assay Type: Direct EIA. Detection Method: Colorimetric Assay. Assay Range: Regular: 0.5 - 32 pmol/ml. Acetylated: 0.3125 - 20 pmol/ml. | Linearity was determined by taking two human urine samples, one with a low cGMP level of 0.3 pmol/mL and one with a higher level of 9.7 pmol/mL, and mixing them in ratios. The measured concentrations were compared to the expected values based on the ratios used. | Chemical structure of cGMP (3',5'-cyclic GMP) Graph of the Regular Format Typical Standard Curve for the Cyclic GMP EIA Kit StressXpress - SKT-211 | Graph of the Acetylated Format Typical Standard Curve for the Cyclic GMP EIA Kit StressXpress - SKT-211 | Graph of the Linearity Recovery for the Cyclic GMP EIA Kit StressXpress - SKT-211 | Chemical Structure of Cyclic GMP for the Cyclic GMP EIA Kit StressXpress - SKT-211
  • Warnings
    Non-hazardous
  • Country of production
    Canada
  • Total weight (kg)
    2
  • Net weight (g)
    300
  • Description
    This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
  • Properties
    MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
  • Gene target
  • Short name
    Cyclic GMP EIA Kit
  • Technique
    EIA, eia
  • Alternative name
    Cyclic GMP EIA reagent
  • Alternative technique
    elisas
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, this GO :0000187 and activation of MAPK activity and biological process this GO :0001541 and ovarian follicle development and biological process this GO :0001669 and acrosomal vesicle and cellular component this GO :0002020 and protease binding and molecular function this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0002320 and lymphoid progenitor cell differentiation and biological process this GO :0002327 and immature B cell differentiation and biological process this GO :0002371 and dendritic cell cytokine production and biological process this GO :0002551 and mast cell chemotaxis and biological process this GO :0002573 and myeloid leukocyte differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0004716 and receptor signaling protein tyrosine kinase activity and molecular function this GO :0005020 and stem cell factor receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006687 and glycosphin this GO lipid metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007283 and spermatogenesis and biological process this GO :0007286 and spermatid development and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008354 and germ cell migration and biological process this GO :0008360 and regulation of cell shape and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008584 and male this GO nad development and biological process this GO :0009314 and response to radiation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009898 and cytoplasmic side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016772 and transferase activity, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0019827 and stem cell maintenance and biological process this GO :0019955 and cytokine binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030032 and lamellipodium assembly and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030217 and T cell differentiation and biological process this GO :0030218 and erythrocyte differentiation and biological process this GO :0030318 and melanocyte differentiation and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0031274 and positive regulation of pseudopodium assembly and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0032762 and mast cell cytokine production and biological process this GO :0035019 and somatic stem cell maintenance and biological process this GO :0035162 and embryonic hemopoiesis and biological process this GO :0035234 and ectopic germ cell programmed cell death and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0035855 and megakaryocyte development and biological process this GO :0038093 and Fc receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038109 and Kit signaling pathway and biological process this GO :0038162 and erythropoietin-mediated signaling pathway and biological process this GO :0042127 and regulation of cell proliferation and biological process this GO :0042511 and positive regulation of tyrosine phosphorylation of Stat1 protein and biological process this GO :0042517 and positive regulation of tyrosine phosphorylation of Stat3 protein and biological process this GO :0042523 and positive regulation of tyrosine phosphorylation of Stat5 protein and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042803 and protein homodimerization activity and molecular function this GO :0043069 and negative regulation of programmed cell death and biological process this GO :0043303 and mast cell degranulation and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043410 and positive regulation of MAPK cascade and biological process this GO :0043473 and pigmentation and biological process this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0046427 and positive regulation of JAK-STAT cascade and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048066 and developmental pigmentation and biological process this GO :0048070 and regulation of developmental pigmentation and biological process this GO :0048103 and somatic stem cell division and biological process this GO :0048170 and positive regulation of long-term neuronal synaptic plasticity and biological process this GO :0048565 and digestive tract development and biological process this GO :0048863 and stem cell differentiation and biological process this GO :0050673 and epithelial cell proliferation and biological process this GO :0050910 and detection of mechanical stimulus involved in sensory perception of sound and biological process this GO :0051091 and positive regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060374 and mast cell differentiation and biological process this GO :0070662 and mast cell proliferation and biological process this GO :0097067 and cellular response to thyroid hormone stimulus and biological process this GO :0097324 and melanocyte migration and biological process this GO :0097326 and melanocyte adhesion and biological process, transferase activity, this GO :0002020: protease binding and also this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0004716: receptor signaling protein tyrosine kinase activity and also this GO :0005020: stem cell factor receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, transferring phosphorus-containing groups and also this GO :0019955: cytokine binding and also this GO :0042803: protein homodimerization activity and also this GO :0046872: metal ion binding, this GO :0002020: protease binding, this GO :0004672: protein kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0004716: receptor signaling protein tyrosine kinase activity, this GO :0005020: stem cell factor receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferring phosphorus-containing groups, this GO :0019955: cytokine binding, this GO :0042803: protein homodimerization activity, this GO :0046872: metal ion binding, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
1. Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
  • Tree numbers
    • E05.478.566.350
    • E05.478.583.400
    • E05.601.470.350
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
Additional information
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