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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana HSP23.5, UniProt: Q9FGM9, TAIR: AT5G51440
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum in PBS pH 7.4.
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How to reconstitute
For reconstitution add 25 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibodyAS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibodyAS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7collection of antibodies to plant heat shock proteins
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Recommended dilutions for use
1: 500 (WB)
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Molecular weight expected аpparent
23.5 kDa
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Verified reactivity
Arabidopsis thaliana recombinant HSP23.5
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Possible reactivity
Arabidopsis thaliana
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No reactivity
Chlamydomonas reinhardtii
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Supplementary information
Please, note that there might be no HSPs accumulation below temperature of 32-34°C. HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration. In this case the HSPs induction requires higher temperatures.
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References
To be added when available, antibody released in September 2016.
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Scientific context
HSP23.5 | heat shock protein 23.5 (mitochondrial) belongs to a small heat shock protein family HSP20.
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Notes
This antibody is recognizing recombinant HSP23.5 and reactivity on endogenous protein needs to be confirmed. High sensitivity reagents and enrichment are necessary due to low amounts of HSP23.5 in endogenous tissue.
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Protein number
Q9FGM9
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TAIR number
AT5G51440
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