Recombinant Burkholderia mallei Biotin synthase (bioB)

Products_type

Recombinant Protein
Products_short_name

[Biotin synthase (bioB)]
Other_names

[biotin synthase; Biotin synthase; biotin synthase]
Products_gene_name

[bioB]
Products_gene_name_syn

[bioB]
Other_gene_names

[bioB; bioB]
Purity

>85% (SDS-PAGE)
Form

Lyophilized or liquid (Format to be determined during the manufacturing process)
Storage_stability

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Burkholderia mallei synthase bioB
Short name

Recombinant Burkholderia mallei synthase (bioB)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

E Coli or Yeast or Baculovirus or Mammalian Cell
Label

Biotin
Alternative name

Rec. Burkholderia mallei biotinilated synthase (bioB)
Alternative technique

rec

Name

Radioimmunosorbent Test
Concept

Scope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data