HotStart DNA Polymerase (PCR)
PCR, RT-PCR & qPCR
PCR. For others please contact us
Refer to NCBI PubMed for current references.
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Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. it can be antibody based or chemical blocked. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity.
PCR, polymerase chain reaction
TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
1. Gene info
Long gene name
DNA polymerase kappa
Synonyms gene name
- polymerase (DNA directed) kappa
- polymerase (DNA) kappa
Entrez gene record
Scope note:Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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