2X PCR Taq MasterMix

  • Catalog number
    G013
  • Product name
    2X PCR Taq MasterMix
  • Size
    5.0 ml (200 Rxns)
  • Price
    97.15
  • Supplier
    ABM PCR reagents
  • Product category
    PCR, RT-PCR & qPCR
  • Application
    PCR. For others please contact us
  • Literature
    Refer to NCBI PubMed for current references.
  • Technical datasheet
    Please contact us to receive a datasheet or any other useful technical documentation available.
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Gene target
  • Short name
    2X PCR Taq MasterMix
  • Technique
    PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. ABM PCR reagents TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
  • Alternative name
    2X PCR test reagent Taq MasterMix
  • Alternative technique
    dna-amplification
MeSH Data
  • Name
  • Concept
    Scope note:In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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