Goat, L), HRP conjugated

• Catalog number
  AS16 3127
• Price
  Please ask
• Size
  1 ml

• Available ordering format
  Lyophilized in PBS, pH 7.2
• Immunogen
  Purified normal IgG isolated from pooled dog serum
• Raised in
  Goat
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Protein G purified IgG
• How to reconstitute
  For reconstitution please add 1 ml of sterile destilled water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Reconstituted material can be stoored in +4°C up to 7 days.
• Verified applications
  dot-blot (Dot), ELISA (ELISA), immunocytochemistry (ICC), immunohistochemistry (paraffin) (IHC), western blot (WB)
• Connected products
  other HRP conjugated secondary antibodies to dog IgGSecondary antibodies
• Recommended dilutions for use
  1:1000 - 1:10 000 (ELISA), 1:100 and 1:500 (ICC, IHC)The optimal working dilution should be established by titration by the end user. Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal.
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  dog IgG
• Possible reactivity
  to be determined
• No reactivity
  This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
• Supplementary information
  Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
• References
  to be added when available, antibody released in January 2016.
• Scientific context
  Goat anti-Dog IgG (H&L), HRP conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
• Notes
  None
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Latin name
  Capra aegagrus hircus
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated

• Gene target
  conjugated  
• Short name
  Goat, L), conjugated
• Technique
  Goat, antibody Conjugates, goats
• Host
  Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
• Label
  HRP
• Species
  Goats
• Alternative name
  caprine, L), horseradish peroxidase coupled
• Alternative technique
  caprine

Similar products