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Chemical available in other sizes
Please inquire size and price
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Stock availability
In Stock
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Cas number
1239-45-8
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Chemical s molecular weight
394.31
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Chemical s main applications
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
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Chemical s category
Amines, Aromatics, Pharmaceuticals, Intermediates & Fine Chemicals
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Other name
3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide; 2,7-Diamino-10-ethyl-9- phenylphenanthridinium Bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium Bromide; 2,7-Diamino-9-phenylphenanthridine Ethobromide; Dromilac; Homidium Bromide;
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Chemical s formula
C21H20BrN3
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Physical properties
Very Dark Red to Dark Purple Solid
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Melting temperature
242-244°C
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Boiling temperature
No Data Available
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Chemical s soluble in
DMSO (Slightly), Methanol (Slightly), Water (Slightly, Sonicated)
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Stability conditions
No Data Available
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Storage
Hygroscopic, Refrigerator, under inert atmosphere
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Description
Ethidium Bromide C21H20BrN3 CAS 1239-45-8 is used for detection of DNA in electrophoresis. Be careful not to contaminate your laboratory wit EtBr as it is genotoxic. The best way to detect Ethidium Bromide contamination is to use a UV black light. The EtBr stain can be safely removed with bleach of sodium hypochlorite, NAOCl that will destroy the Ethidium. GENTAUR's EtBr Destroyer Bag or sprayer will reduce it to a non mutagenic non fluorescent solution.