Sphingomyelin Quantification Colorimetric Assay Kit

• Catalog number
  K600-100
• Price
  Please ask
• Size
  100 assays

• Description
  The most sensitive kit on the market
• Summary
  • Detection method- Absorbance (OD 570 nm) • Applications- - Sphingomyelin quantification in various biological samples - Analysis of cell signaling pathway
• Detection Method
  Absorbance (OD 570 nm)
• Applications
  • Sphingomyelin quantification in various biological samples • Analysis of cell signaling pathway
• Sample Type
  • Serum, plasma • Tissue samples such as brain etc. • Adhesion or suspension cells
• Features Benefits
  • Simple, rapid & convenient • The assay can detect sphingomylein less than 1 µM in a variety of samples
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Sphingomyelin is an important structural lipid component of cell membranes and lipoproteins. Sphingomyelin has been implicated in the pathogenesis of several diseases; therefore a sensitive and reliable technique for its quantification is of considerable importance. BioVision’s Sphingomyelin Quantification Assay Kit provides a simple and sensitive method for quantifying sphingomyelin using colorimetry. In the assay, sphingomyelin is hydrolyzed into ceramide and phosphorylcholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphorylcholine to choline, which then reacts with Sphingomyelin Enzyme Mix to produce an intermediate. The intermediate reacts with a highly specific probe to generate color (OD 570 nm). The kit can detect sphingomyelin in various biological samples as low as 1 µM.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Sphingomyelin   Quantification   Kit  
• Short name
  Sphingomyelin Quantification Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Sphingomyelin Quantification colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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