Quick Cell Proliferation colorimetric Assay Kit Plus

• Catalog number
  K302-2500
• Price
  Please ask
• Size
  2500 assays

• Description
  Sensitive, Non-radioactive Assay
• Summary
  • Detection- Absorbance (420-480 nm) • Species reactivity- Mammalian • Kit size- 500, 2500 • Applications- The assay measures cell proliferation in response to growth factors/cytokines/mitogens/and nutrients etc.
• Sample Type
  Cell cultures (adherent and suspension)
• Features Benefits
  • Simple one-step procedure; takes less than 4 hours • Convenient • Uses a water-soluble tetrazolium salt WST reagent. The method is so simple that requires no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  The Quick Cell Proliferation Assay Kit II provides by far the easiest and most sensitive means for quantifying cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt to formazan by cellular mitochondrial dehydrogenase. The amount of the dye generated by activity of dehydrogenase is directly proportional to the number of living cells. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, just add-and-read, requiring no washing, no harvesting, and no solubilization steps. It is faster, stable, and more sensitive than MTT, XTT, MTS based assays. The assay correlates well with the [3H]-thymidine incorporation assay.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Quick   Proliferation   Kit   Plus  
• Short name
  Quick Proliferation colorimetric Assay Kit Plus
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Quick cellular expantion colorimetry test reagent Plus
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

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