OxiSelect™ Hydrogen Peroxide/Peroxidase Assay test kit (Fluorimetry), Trial Size
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Catalog number
STA-344-T
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Price
Please ask
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Size
50 assays
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We send this reagant on
Room temperature
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The temperatureerature for storage is
Upon receipt, aliquot and store the ADHP probe, HRP at -20ºC. Avoid multiple freeze/thaw cycles. Store the remaining test kit components at 4ºC. Protect ADHP from light.
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, Reactive Oxygen Species (ROS) Assays, Hydrogen Peroxide and Peroxidase Assays
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Use of the reagent
The OxiSelect™ Hydrogen Peroxide/Peroxidase Assay test kit is a sensitive quantitative Fluorimetry assay for hydrogen peroxide or peroxidase. In the presence of HRP, ADHP reacts with H2O2 in a 1:1 stoichiometry to produce highly fluorescent Resorufin. The Resorufin product can be easily read by a fluorescence microplate reader with an excitation of 530-560 nm and an emission of 590 nm, or for absorbance at 560 nm. Fluorescence values are proportional to the H2O2 or peroxidase levels within the samples. The H2O2 or peroxidase content in unknown samples is determined by comparison with its respective standard curve.
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Cellular use
Detect hydrogen peroxide concentrations as low as 50 nM
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Protocol information
Suitable for use with cell lysates, tissue homogenates, cell culture supernatants, plasma, serum, urine, or other biological fluids
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Reagent properties
Simple assay protocol provides results in 30-90 minutes, depending on sample type
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,92
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Test
Cellular biology labs supplies other types of Assays as 1.
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Conjugate
Peroxidases with EC number 1.11.1.x are catalyzing the reaction of
ROOR' + electron donor (2 e−) + 2H+ → ROH + R'OH
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Gene target
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Short name
OxiSelect™ Hydrogen Peroxide/ Assay test kit (Fluorimetry), Trial Size
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Technique
Assay, assays
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Host
Assay
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Label
peroxidase
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Alternative name
OxiSelect™ Hydrogen Peroxide/Peroxidase test test reagent (Fluorimetry), Trial Size
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Alternative technique
arrays, tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
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Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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