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Category
Primary Antibodies
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Long description
The defined antibody reactivity is restricted to VWF. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony) against normal plasma, a single precipitin line is obtained which shows a reaction of identity with precipitated purified FVIII/VWF. No precipitation is obtained with plasma of type 1 congenital Von Willebrand’s disease. It is strongly recommended to perform the EID-assay with the special agarose (Agarose Nordic nr.4) Nordic High Resolution Buffer and High resolution-buffered Agarose, performance tested to assure reliable and reproducible results. The threshold of detection in this technique is about 0.15 mg per 100 ml. Haemophilia A and Von Willebrand’s disease (VWD) are known to be FVIII/VWF deficiencies. Immunologic determination of VWF antigen enables to discriminate between these two bleeding conditions. Haemophilia A patients lack FVIIIC but have a normal level of VWFag. In VWD, both FVIIIC and VWFag are reduced. Active and inactive FVIII/VWF, its breakdown products and inactivated FVIII/VWF-inhibitor complexes all express antigen determinants of VWFag and may be recognized by polyclonal antisera. Determination f plasma VWFag levels in addition to the level of FVIIIC can contribute also to the detection of the carrier state in haemophilia A. While the level of VWFag is normal or even elevated, the average concentration of FVIIIC of carriers is about half of normal. Various types and subtypes of congenital VWD have been described. Cases of acquired VWD have been reported in association with several clinical diseases including autoimmune disease, systemic lupus erythematosus, benign monoclonal gammapathy and Waldenström’s macroglobulinemia.
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Antibody come from
Human FVIII procoagulant activity (FVIIIC) is carried by a polypeptide non-covalently bound to a large carrier molecule (unit molecular weight 250 kD) known as the Von Willebrand factor (VWF). FVIII/VWF exists in plasma as a series of polymers with molecular weights > 1.100 kD. FVIIIC is probably formed in the liver, is unstable on storage but sufficiently stable in fresh frozen plasma as cryoprecipitate or FVIII concentrate. VWF is synthesized in megakaryocytes and vascular endothelial cells; it is also present in the alpha-granules and membranes of platelets, binding to specific sites on the activated platelet after its release. It is responsible for platelet adhesion to the vascular subendothelium. FVIIIC, but not VWF is completely consumed during coagulation ad is absent from serum. Polyclonal antisera are raised against FVIII/VWF purified from plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. IgG protein concentration in the antiserum is 10 mg/ml. No foreign proteins added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Goat anti human Factor VIII related antigen (VWD) Antibody
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Antibody is raised in
Goat
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Antibody s reacts with
Not applicable
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Antibody s reacts with these species
The antiSerum does not cross-react with any other Human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Goat antiSerum to Human Von Willebrand Factor (FVIII VWF)
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Research interest
Cardiovascular
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Application
not tested yet / to be tested
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Antibody s suited for
The antiserum concentration required in the gel is normally between 1 and 2%. In immunologic determinations of FVIII/VWF, plasma samples and all assay components must contain EDTA to stabilize the proteins The antiserum is standardized for use in the electroimmunodiffusion (EID, Laurell) test procedure for the quantitative determination of FVIII/VWF as scribed in the Nordic Recommended Working Procedure.
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Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk. Antigens are peptides or recombinant or native dependent on the production method. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Latin name
Capra aegagrus hircus
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
