Chimeric anti-Ebola Virus GP Monoclonal Antibody (h13F6)
A murine / human chimeric IgG produced in N. benthamiana and is reactive to EBOV GP. The antibody detects GP in virus-like particles (VLP) and recombinant GP without the transmembrane region (rGPdTM).
Ebola Virus (EBOV)
Venezuelan equine encephalitis virus
Enzyme-Linked Immunosorbent Assay, Western Blot
2-3 weeks +4C, -20C long term
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by virus detection they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Marburg or Ebola viruses ( EBOV ) need to be handled in BSL4 Biological safety level 4. However the monoclonal and polyclonal antibodies, ELISAs and recombinant proteins do not need any specific safety measure because no active virus is involved. The Zaire Ebola strain is called ZEBOV. The Sudan virus is called SEBOV.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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