Mouse Anti-Rat GABA-A Receptor Delta Monoclonal IgG2a, Clone S151-3

• Catalog number
  SMC-345D-A594
• Price
  Please ask
• Size
  100 µg

• Clone
  S151-3
• Immunogen
  Synthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
• Antibody s full description
  Mouse Anti-Rat GABA A Receptor Monoclonal IgG2a Antibody, Clone: S151-3: ATTO 594
• Antibody s category
  Monoclonal Antibodies
• Antibody s other name
  GABAA-RD Antibody, Gabrd Antibody, MGC105467 Antibody
• Raised in
  Mouse
• Antibody s target
  GABA A Receptor
• Primary research fields
  Neuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
• Brandname
  None
• Antibodies applications
  WB, ICC/IF, AM
• Antibody s reactivity
  Human, Mouse, Rat
• Antibody s dilutions
  WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Purity
  Protein G Purified
• Antibody buffer for storage
  PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Antibody s concentration
  1 mg/ml
• Antibody s specificity
  Detects ~55kDa.
• Storage recommendations
  -20ºC
• Shipping recommendations
  Blue Ice or 4ºC
• Antibody certificate of analysis
  2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Antibody in cell
  Cell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
• Tissue specificity
  See included datasheet or contact our support service
• Scientific context
  The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
• Bibliography
  1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
• Released date
  13-Apr-2010
• Tested applications
  To be tested
• Tested reactivity
  To be tested
• NCBI number
  NP_058985.1
• Gene number
  29689
• Protein number
  P18506
• Antibody s datasheet
  Contact our support service
• Representative figure link
  more | details
• Representative figure legend
  Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (SMC-345). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-345) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei. Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (SMC-345). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-345) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-GABA A Receptor Antibody [S151-3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-345) Mouse Anti-GABA A Receptor Antibody [S151-3] used in Western Blot (WB) on Rat Cell line lysates (SMC-345)
• Warning information
  Non-hazardous
• Country of production
  Canada
• Total weight kg
  1.4
• Net weight g
  0.1
• Stock availability
  In Stock
• Other related products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus, Rattus norvegicus

• Gene target
  GABA-A   Receptor   Delta   Monoclonal   IgG2a   S151-3  
• Gene symbol
  TSPAN3, ABAT, HLA-A, IGHV5-10-1, CXCL1, CIDEA, RPA1, CDC37P1, OR1Q1
• Short name
  Mouse Anti- GABA-A Receptor Delta Monoclonal IgG2a, Clone S151-3
• Technique
  anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
• Host
  mouse
• Isotype
  IgG2a, IgG2a
• Label
  ATTO 594
• Species
  Rat, Mouses, Rats
• Alternative name
  Mouse Antibody toRat GABA-A Receptor Delta monoclonal IgG2a, clonality S151-3
• Alternative technique
  murine, antibodies
• Clone name
  S151-3

Gene info

• Identity
  HGNC:17752
• Gene
  TSPAN3
• Long gene name
  tetraspanin 3
• Synonyms gene
  • TM4SF8
• Synonyms gene name
  • transmembrane 4 superfamily member 8
• Synonyms
  • TM4-A
  • TSPAN-3
• Locus
  15q24.3
• Discovery year
  2003-07-21
• Entrez gene record
  10099
• RefSeq identity
  • NM_005724
• Classification
  • Tetraspanins
• VEGA ID
  OTTHUMG00000143728

Gene info

• Identity
  HGNC:23
• Gene
  ABAT
• Long gene name
  4-aminobutyrate aminotransferase
• Synonyms
  • GABAT
  • GABA-T
• Synonyms name
  • 4-aminobutyrate transaminase
  • gamma-aminobutyrate aminotransferase
  • GABA transaminase
• GenBank acession
  • L32961
• Locus
  16p13.2
• Discovery year
  1996-03-13
• Entrez gene record
  18
• Pubmed identfication
  • 7721088
• RefSeq identity
  • NM_020686
• VEGA ID
  OTTHUMG00000048201

Gene info

• Identity
  HGNC:4931
• Gene
  HLA-A
• Long gene name
  major histocompatibility complex, class I, A
• GenBank acession
  • D32129
• Locus
  6p22.1
• Discovery year
  2001-06-22
• Entrez gene record
  3105
• Pubmed identfication
  • 8838351
• RefSeq identity
  • NM_002116
• Classification
  • C1-set domain containing
  • Histocompatibility complex
• VEGA ID
  OTTHUMG00000130501
• Locus Specific Databases
  • IMGT/HLA Database

Gene info

• Identity
  HGNC:5661
• Gene
  IGHV5-10-1
• Long gene name
  immunoglobulin heavy variable 5-10-1
• Synonyms gene
  • IGHV5-A
• Synonyms gene name
  • immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
  • immunoglobulin heavy variable 5-A (provisional)
• Synonyms
  • IGHV5-a
• GenBank acession
  • X92227
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28386
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000191143

Gene info

• Identity
  HGNC:4602
• Gene
  CXCL1
• Long gene name
  C-X-C motif chemokine ligand 1
• Synonyms gene
  • MGSA
  • GRO1
  • FSP
• Synonyms gene name
  • GRO1 oncogene (melanoma growth stimulating activity, alpha)
  • fibroblast secretory protein
  • chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Synonyms
  • SCYB1
  • GROa
  • MGSA-a
  • NAP-3
• Synonyms name
  • melanoma growth stimulating activity, alpha
• GenBank acession
  • J03561
• Locus
  4q13.3
• Discovery year
  1988-08-11
• Entrez gene record
  2919
• Pubmed identfication
  • 2217207
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000160866

Gene info

• Identity
  HGNC:1976
• Gene
  CIDEA
• Long gene name
  cell death inducing DFFA like effector a
• Synonyms gene name
  • cell death-inducing DFFA-like effector a
• Synonyms
  • CIDE-A
• GenBank acession
  • AF041378
• Locus
  18p11.21
• Discovery year
  1998-07-15
• Entrez gene record
  1149
• Pubmed identfication
  • 9564035
  • 18509062
• RefSeq identity
  • NM_001279
• VEGA ID
  OTTHUMG00000131691

Gene info

• Identity
  HGNC:10289
• Gene
  RPA1
• Long gene name
  replication protein A1
• Synonyms gene name
  • replication protein A1 (70kD)
  • replication protein A1, 70kDa
• Synonyms
  • REPA1
  • RPA70
  • HSSB
  • RF-A
  • RP-A
• GenBank acession
  • M63488
• Locus
  17p13.3
• Discovery year
  1993-12-14
• Entrez gene record
  6117
• Pubmed identfication
  • 8454588
• RefSeq identity
  • NM_002945
• Classification
  • Nucleotide excision repair
• VEGA ID
  OTTHUMG00000090579

Gene info

• Identity
  HGNC:34406
• Gene
  CDC37P1
• Long gene name
  cell division cycle 37 pseudogene 1
• Synonyms gene name
  • cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
• Synonyms
  • A-761H5.2
• Locus
  16p11.2
• Discovery year
  2009-01-29
• Entrez gene record
  390688
• Pubmed identfication
  • 15616553
• RefSeq identity
  • NG_009463
• VEGA ID
  OTTHUMG00000175739

Gene info

• Identity
  HGNC:8223
• Gene
  OR1Q1
• Long gene name
  olfactory receptor family 1 subfamily Q member 1
• Synonyms gene
  • OR1Q2
  • OR1Q3
• Synonyms gene name
  • olfactory receptor, family 1, subfamily Q, member 1
• Synonyms
  • OST226
  • OR9-A
  • HSTPCR106
  • OST226OR9-A
  • TPCR106
• Locus
  9q33.2
• Discovery year
  1999-12-09
• Entrez gene record
  158131
• Classification
  • Olfactory receptors, family 1
• VEGA ID
  OTTHUMG00000020615

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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