Mouse Anti-Rat GABA-A Receptor Delta Monoclonal IgG2a, Clone S151-3
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Catalog numberSMC-345D-A390
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PricePlease ask
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Size100 µg
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CloneS151-3
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ImmunogenSynthetic peptide amino acids 15-34 of rat GABA-A-R-Delta
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Antibody s full descriptionMouse Anti-Rat GABA A Receptor Monoclonal IgG2a Antibody, Clone: S151-3: ATTO 390
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Antibody s categoryMonoclonal Antibodies
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Antibody s other nameGABAA-RD Antibody, Gabrd Antibody, MGC105467 Antibody
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Raised inMouse
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Antibody s targetGABA A Receptor
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Primary research fieldsNeuroscience, Neurotransmitter Receptors, GABA Receptors, GABAA Receptors
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BrandnameNone
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Antibodies applicationsWB, ICC/IF, AM
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Antibody s reactivityHuman, Mouse, Rat
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Antibody s dilutionsWB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
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PurityProtein G Purified
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Antibody buffer for storagePBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration1 mg/ml
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Antibody s specificityDetects ~55kDa.
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Storage recommendations-20ºC
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Shipping recommendationsBlue Ice or 4ºC
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Antibody certificate of analysis2 µg/ml of SMC-345 was sufficient for detection of Delta1 GABA-A receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cellCell Membrane , Cell Junction , Synapse , Postsynaptic Cell Membrane
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Tissue specificitySee included datasheet or contact our support service
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Scientific contextThe GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2).
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Bibliography1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136. 2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
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Released date13-Apr-2010
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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NCBI numberNP_058985.1
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Gene number29689
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Protein numberP18506
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Antibody s datasheetContact our support service
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Representative figure link
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Representative figure legendImmunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (SMC-345). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-345) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Diffuse cytoplasm and dull nuclei. Western Blot analysis of Rat Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S151-3 (SMC-345). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody (SMC-345) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-GABA A Receptor Antibody [S151-3] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human HaCaT cells (SMC-345) Mouse Anti-GABA A Receptor Antibody [S151-3] used in Western Blot (WB) on Rat Cell line lysates (SMC-345)
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilityIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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Gene target
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Gene symbolTSPAN3, ABAT, HLA-A, IGHV5-10-1, CXCL1, CIDEA, RPA1, CDC37P1, OR1Q1
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Short nameMouse Anti- GABA-A Receptor Delta Monoclonal IgG2a, Clone S151-3
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Techniqueanti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
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Hostmouse
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IsotypeIgG2a, IgG2a
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LabelATTO 390
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SpeciesRat, Mouses, Rats
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Alternative nameMouse Antibody toRat GABA-A Receptor Delta monoclonal IgG2a, clonality S151-3
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Alternative techniquemurine, antibodies
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Clone nameS151-3
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Gene info
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Identity
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Gene
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Long gene nametetraspanin 3
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Synonyms gene
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Synonyms gene name
- transmembrane 4 superfamily member 8
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Synonyms
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Locus
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Discovery year2003-07-21
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Entrez gene record
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RefSeq identity
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Classification
- Tetraspanins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name4-aminobutyrate aminotransferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1996-03-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameC-X-C motif chemokine ligand 1
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Synonyms gene
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Synonyms gene name
- GRO1 oncogene (melanoma growth stimulating activity, alpha)
- fibroblast secretory protein
- chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-11
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Entrez gene record
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Pubmed identfication
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell division cycle 37 pseudogene 1
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Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
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Synonyms
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Locus
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Discovery year2009-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily Q member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily Q, member 1
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Synonyms
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Classification
- Olfactory receptors, family 1
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data