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Clone
2G4
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Immunogen
Synthetic peptide from the N-terminal of Rat ENaC alpha (aa. 46-68)
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Antibody s full description
Mouse Anti-Rat ENaC alpha Monoclonal IgG2b Antibody, Clone: 2G4: ATTO 390
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Antibody s category
Monoclonal Antibodies
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Antibody s other name
SCNN1A Antibody, Epithelial Sodium Channel-α Antibody, Epithelial Sodium Channel alpha Antibody, Alpha ENaC 2 Antibody, Alpha ENaC Antibody, Alpha NaCH Antibody, Alpha-ENaC Antibody, Amiloride sensitive epithelial sodium channel alpha subunit Antibody, Amiloride sensitive sodium channel subunit alpha Antibody, Amiloride-sensitive sodium channel subunit alpha Antibody, ENaCa Antibody, ENaCalpha Antibody, Epithelial Na(+) channel subunit alpha Antibody, Epithelial Na+ channel subunit alpha Antibody, FLJ21883 Antibody, Nonvoltage gated sodium channel 1 subunit alpha Antibody, Nonvoltage-gated sodium channel 1 subunit alpha Antibody, SCNEA Antibody, SCNN 1 Antibody, SCNN1 Antibody, SCNN1A Antibody, SCNNA_HUMAN Antibody, Sodium channel nonvoltage gated 1 alpha Antibody
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Raised in
Mouse
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Antibody s target
ENaC alpha
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Primary research fields
Neuroscience, Ion Channels, Sodium Channels, Epithelial Sodium Channels (ENaC)
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Brandname
None
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Antibodies applications
WB, IHC, IF
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Antibody s reactivity
Mouse, Rat
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Antibody s dilutions
WB (1:1000); IHC (1:150); optimal dilutions for assays should be determined by the user.
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Purity
Protein G Purified
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Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
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Antibody s concentration
1 mg/ml
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Antibody s specificity
Detects ~85kDa.
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Storage recommendations
-20ºC
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Shipping recommendations
Blue Ice or 4ºC
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Antibody certificate of analysis
A 1:1000 dilution of SMC-239 was sufficient for detection of ENaC alpha in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
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Antibody in cell
Apical Cell Membrane
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Tissue specificity
See included datasheet or contact our support service
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Scientific context
The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport (1). Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption (2). ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta, and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate (3, 4). The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure (6).
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Bibliography
1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128.
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Released date
12-Sep-2016
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Tested applications
To be tested
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Tested reactivity
To be tested
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NCBI number
NP_113736
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Gene number
25122
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Protein number
Q6IRJ1
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Antibody s datasheet
Contact our support service
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Representative figure link
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Representative figure legend
Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4 (SMC-239). Tissue: Kidney. Species: Rat. Fixation: Paraffin-embedded formalin-fixed. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (SMC-239) at 1:100. Secondary Antibody: Goat Anti-Mouse ATTO 488 (green). Localization: Intercalated cells. Aquaporin 2 Antibody staining in red. Immunohistochemistry analysis using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4 (SMC-239). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (SMC-239) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X. Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~85kDa ENaC alpha protein using Mouse Anti-ENaC alpha Monoclonal Antibody, Clone 2G4 (SMC-239). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC alpha Monoclonal Antibody (SMC-239) at 1:1000. Predicted/Observed Size: ~85kDa. Mouse Anti-ENaC alpha Antibody [2G4] used in Immunohistochemistry (IHC) on Rat Kidney (SMC-239) Mouse Anti-ENaC alpha Antibody [2G4] used in Immunohistochemistry (IHC) on Mouse Kidney (cortex) (SMC-239) Mouse Anti-ENaC alpha Antibody [2G4] used in Western Blot (WB) on Mouse Whole kidney homogenates (SMC-239)
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availability
In Stock
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Description
The Mouse Anti- ENaC alpha Monoclonal IgG2b, Clone 2G4 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus