Alternative name 1
CD8a, T-Cell, Cytotoxic/Suppressor, NK Cell, Thymocyte, Intestinal Intraepithelial Lymphocyte, Clone: OX-8, Mab anti-Rat, FITC
Alternative name 2
CD8a, T-Cell, Cytotoxic/Suppressor, NK Cell, Thymocyte, Intestinal Intraepithelial Lymphocyte, Clone: OX-8, Mab antibody-Rat, FITC
Alternative name 3
CD8a, T-Cell, Cytotoxic/Suppressor, NK Cell, Thymocyte, Intestinal Intraepithelial Lymphocyte, Clone: OX-8, Mab antibody-Rattus norvegicustus norvegicus, FITC
Alternative name 4
anti-CD8a T-Cell Cytotoxic/Suppressor NK Cell Thymocyte Intestinal Intraepithelial Lymphocyte Clone: OX-8
Alternative name 5
CD8a T-Cell Cytotoxic/Suppressor NK Cell Thymocyte Intestinal Intraepithelial Lymphocyte Clone: OX-8
This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
Storage and handling
Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
For cells, cell lines and tissues in culture till half confluency. Kinase, breast cancer tumor, HRAS like - suppressor protein make genetic suppression restoring the phenotype seen prior to the original background in biological pathways.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus, Rattus norvegicus
CD8a, T- Cytotoxic/Suppressor, NK Thymocyte, Intestinal Intraepithelial Lymphocyte, Clone: OX-8, Mouse Monoclonal antibody- FITC
Mouse monoclonal, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
Rat, Mouses, Rats
CD8a molecule, T-cellular, Cytotoxic/Suppressor, NK cellular, Thymocyte, Intestinal Intraepithelial Lymphocyte, clonality: OX-8, Mouse monoclonal (Antibody to)-Rat, fluorecein
Alternative to gene target
CD8a molecule, CD8 and Leu2 and MAL and p32, CD8A and IDBG-59987 and ENSG00000153563 and 925, this GO :0002456 and T cell mediated immunity and biological process this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0015026 and coreceptor activity and molecular function this GO :0016020 and membrane and cellular component this GO :0019882 and antigen processing and presentation and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042288 and MHC class I protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0045065 and cytotoxic T cell differentiation and biological process this GO :0045087 and innate immune response and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0051607 and defense response to virus and biological process, protein homodimerization activity, this GO :0005515: protein binding and also this GO :0015026: coreceptor activity and also this GO :0019901: protein kinase binding and also this GO :0042288: MHC class I protein binding and also this GO :0042803: protein homodimerization activity, this GO :0005515: protein binding, this GO :0015026: coreceptor activity, this GO :0019901: protein kinase binding, this GO :0042288: MHC class I protein binding, this GO :0042803: protein homodimerization activity, Extracellular, Cd8a and IDBG-155582 and ENSMUSG00000053977 and 12525, CD8A and IDBG-634911 and ENSBTAG00000021141 and 281060