CD4, T-Cell, Helper, Clone: W3/25, Mouse Monoclonal antibody-Rat, Biotin, frozen/paraffin

  • Catalog number
    ACL003B
  • Product name
    CD4, T-Cell, Helper, Clone: W3/25, Mouse Monoclonal antibody-Rat, Biotin, frozen/paraffin
  • Size
    0.1mg
  • Price
    249.99
  • Supplier
    accurate-monoclonals
  • Alternative name 1
    CD4, T-Cell, Helper, Clone: W3/25, Mab anti-Rat, Biotin, frozen/paraffin
  • Alternative name 2
    CD4, T-Cell, Helper, Clone: W3/25, Mab antibody-Rat, Biotin, frozen/paraffin
  • Alternative name 3
    CD4, T-Cell, Helper, Clone: W3/25, Mab antibody-Rattus norvegicustus norvegicus, Biotin, frozen/paraffin
  • Alternative name 4
    anti-CD4 T-Cell Hel Clone: W3/25
  • Alternative name 5
    CD4 T-Cell Hel Clone: W3/25
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Additional description
    CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. For cells, cell lines and tissues in culture till half confluency.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Short name
    CD4, T- Helper, Clone: W3/25, Mouse Monoclonal antibody- , frozen/paraffin
  • Technique
    Mouse monoclonal, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Label
    Biotin
  • Species
    Rat, Mouses, Rats
  • Alternative name
    CD4 molecule, T-cellular, Helper, clonality: W3/25, Mouse monoclonal (Antibody to)-Rat, biotinilated, -25°C frozen/paraffin
  • Alternative technique
    antibodies
  • Alternative to gene target
    CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, this GO :0001816 and cytokine production and biological process this GO :0001948 and glycoprotein binding and molecular function this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005201 and extracellular matrix structural constituent and molecular function this GO :0005515 and protein binding and molecular function this GO :0005769 and early endosome and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006948 and induction by virus of host cell-cell fusion and biological process this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007167 and enzyme linked receptor protein signaling pathway and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0015026 and coreceptor activity and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030217 and T cell differentiation and biological process this GO :0030260 and entry into host cell and biological process this GO :0031295 and T cell costimulation and biological process this GO :0032507 and maintenance of protein location in cell and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042289 and MHC class II protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0045058 and T cell selection and biological process this GO :0045086 and positive regulation of interleukin-2 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045234 and protein palmitoleylation and biological process this GO :0045860 and positive regulation of protein kinase activity and biological process this GO :0050690 and regulation of defense response to virus by virus and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0050863 and regulation of T cell activation and biological process this GO :0050870 and positive regulation of T cell activation and biological process, protein homodimerization activity, this GO :0001948: glycoprotein binding and also this GO :0004872: receptor activity and also this GO :0004888: transmembrane signaling receptor activity and also this GO :0005201: extracellular matrix structural constituent and also this GO :0005515: protein binding and also this GO :0008270: zinc ion binding and also this GO :0015026: coreceptor activity and also this GO :0019899: enzyme binding and also this GO :0019901: protein kinase binding and also this GO :0042289: MHC class II protein binding and also this GO :0042803: protein homodimerization activity, this GO :0001948: glycoprotein binding, this GO :0004872: receptor activity, this GO :0004888: transmembrane signaling receptor activity, this GO :0005201: extracellular matrix structural constituent, this GO :0005515: protein binding, this GO :0008270: zinc ion binding, this GO :0015026: coreceptor activity, this GO :0019899: enzyme binding, this GO :0019901: protein kinase binding, this GO :0042289: MHC class II protein binding, this GO :0042803: protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
MeSH Data
  • Name
  • Concept
    Scope note:The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.
  • Tree numbers
    • E01.370.225.500.620.720.610
    • E01.370.225.750.600.720.610
    • E05.200.500.620.720.610
    • E05.200.750.600.720.610
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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