Donkey anti Rat IgG H + L Alexa Fluor 594
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Catalog number43R-ID047AF
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PricePlease ask
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Size500 µg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenDonkey anti-rat IgG (H + L) (Alexa Fluor 594) was raised in donkey using Rat IgG (H&L) ..
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Raised inDonkey
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SpecificityRat whole IgG
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Cross ReactivityMinimal crossreaction with Human, Goat, Bovine, Rabbit, Guniea Pig, Sheep, Syrian Hamster, Mouse, Chicken, Horse
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Conjugate TagAlexa Fluor 594
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Method of PurificationDonkey anti Rat IgG (H + L) (Alexa Fluor 594) was purified by affinity chromatography.
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Concentration1.5 mg/ml
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Form BufferLyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
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StorageStore lyophilized at 4 deg C. Reconstitute in 400 ul dH2O, and store for up to 6 weeks at -20 deg C or aliquot and store at -70 deg C long term.
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Shipping conditionsBlue Ice
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Tested forWB; IFA; IF; ELISA
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Usage RecommendationsUse at 1:100-1:800 for most applications
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PropertiesFor facs or microscopy Alexa 1 conjugate.
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ConjugationAlexa Fluor, ALEXA FLUOR® 594
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ConjugatedAlexa conjugate 1
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin nameEquus africanus asinus, Rattus norvegicus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target-
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Gene symbolCALD1, FUT1, L3MBTL2
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Short nameanti IgG H + L
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Techniqueanti, IgG, antibody to, IgGs
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HostRat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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IsotypeIgG, IgG
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LabelALEXA FLUOR 594
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SpeciesRat, Donkeys, Rats
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Alternative nameDonkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)
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Alternative techniqueantibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecaldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameL3MBTL histone methyl-lysine binding protein 2
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Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data