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Donkey anti Rat IgG H + L Alexa Fluor 594

  • Catalog number
  • Product name
    Donkey anti Rat IgG H + L Alexa Fluor 594
  • Size
    500 µg
  • Price
  • Supplier
  • Ask
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
  • Type of Immunogen
    Donkey anti-rat IgG (H + L) (Alexa Fluor 594) was raised in donkey using Rat IgG (H&L) ..
  • Raised in
  • Specificity
  • Cross Reactivity
    Minimal reaction with Human, Hamster, Bovine, Guinea Pig, Goat, Chicken, Horse, Rabbit and Sheep serum proteins.
  • Conjugate/Tag
    Alexa Fluor 594
  • Method of Purification
    Donkey anti Rat IgG (H + L) (Alexa Fluor 594) was purified by affinity chromatography.
  • Form & Buffer
    Lyophilized from 0.01M Na3PO4, 0.25M NaCl, pH 7.6, with 15mg/ml BSA, and 0.05% NaN3.
  • Storage
    Store at 4 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; IF; Electrophoresis
  • Usage Recommendations
    1:100 to 1:800
  • Assay Information
    Amax=591; Emax=614
  • Properties
    For facs or microscopy Alexa 1 conjugate.
  • Conjugation
    Alexa Fluor, ALEXA FLUOR® 594
  • Conjugated
    Alexa conjugate 1
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Latin name
    Equus africanus asinus, Rattus norvegicus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Additional isotype
  • Gene target
    H + L
  • Short name
    anti IgG H + L
  • Technique
    anti, antibody to, IgGs
  • Host
    Rat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Isotype
  • Label
  • Species
    Rat, Donkeys, Rats
  • Alternative name
    Donkey antibody to Rat Immunoglobulin G H and L Alexa Fluor 594
  • Alternative technique
  • Ask
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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