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Purified Alpha-Fetoprotein from umbilical cord blood
Human Alpha-Fetoprotein (no cross-reactivity with HSA, CEA, Ferritin, HCG, and PSA)
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Antibody's raised in
Product is supplied as
Recommended concentration for use
1:10 to 1:50
No protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
IHC(C, P), WB
Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
Store at 2-8°C
PGo to UniProt/SwissProt2771 (FETA_HUMAN)
1. MacDonald D.J., and Kelly A.M. (1978) The rapid quantitation of serum alpha-fetoprotein by two-site microenzyme immunoassay. Clin. Chim. Acta 87; 367-372. 2. Jacobsen G.K., Jacobsen M., and Clausen P.P. (1981) Distribution of tumor-associated antigens in the various histologic components of germ cell tumors of the testis. Am. J. Surg. Pathol. 5; 257-266.
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
The Mouse Monoclonal IgG1 Anti-Alpha-Fetoprotein (AFP) [A-013-01/F1-6P2A8-P2B9A9] is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.