Mouse Monoclonal IgG1 Anti-Alpha-Fetoprotein (AFP) [A-013-01/F1-6P2A8-P2B9A9]
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Catalog numberAF100
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PricePlease ask
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Size1 ml
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AvailabilityPlease contact us to check the availability
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ImmunogenPurified Alpha-Fetoprotein from umbilical cord blood
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SpecificityHuman Alpha-Fetoprotein (no cross-reactivity with HSA, CEA, Ferritin, HCG, and PSA)
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Species reactivityHuman
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Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution/test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG1
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Antibody s cloneA-013-01/F1-6P2A8-P2B9A9
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Product is supplied asliquid concentrate
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Recommended concentration for use1:10 to 1:50
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Protein concentrationN/A
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Positive controlFetal liver
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PretreatmentNo protease pre-treatment required, but microwave treatment usually will intensify staining. We refer to BIOLOGO's Unmasking Fluid G (Art. No. DE007).
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Tested applicationsIHC(C, P), WB
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Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage methodStore at 2-8°C
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UniProt numberPGo to UniProt/SwissProt2771 (FETA_HUMAN)
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Litterature1. MacDonald D.J., and Kelly A.M. (1978) The rapid quantitation of serum alpha-fetoprotein by two-site microenzyme immunoassay. Clin. Chim. Acta 87; 367-372. 2. Jacobsen G.K., Jacobsen M., and Clausen P.P. (1981) Distribution of tumor-associated antigens in the various histologic components of germ cell tumors of the testis. Am. J. Surg. Pathol. 5; 257-266.
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Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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DescriptionThe Bioprime is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolAFP, HCG4P1, HLA-A, IGHV5-10-1, CIDEA, RPA1, CDC37P1, OR1Q1, METTL13
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Short nameBioprime
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TechniqueMouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG1
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SpeciesMouse, Mouses
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Alternative nameAFP, Alpha-Fetoprotein
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene namealpha fetoprotein
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-04-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHLA complex group 4 pseudogene 1
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Synonyms
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Locus
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Discovery year2003-12-16
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Entrez gene record
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class I, A
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 5-10-1
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Synonyms gene
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Synonyms gene name
- immunoglobulin heavy variable 5-A (provisional, gene/pseudogene)
- immunoglobulin heavy variable 5-A (provisional)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell death inducing DFFA like effector a
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Synonyms gene name
- cell death-inducing DFFA-like effector a
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namereplication protein A1
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Synonyms gene name
- replication protein A1 (70kD)
- replication protein A1, 70kDa
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Nucleotide excision repair
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecell division cycle 37 pseudogene 1
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Synonyms gene name
- cell division cycle 37 homolog (S. cerevisiae) pseudogene 1
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Synonyms
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Locus
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Discovery year2009-01-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameolfactory receptor family 1 subfamily Q member 1
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Synonyms gene
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Synonyms gene name
- olfactory receptor, family 1, subfamily Q, member 1
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Synonyms
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Locus
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Discovery year1999-12-09
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Entrez gene record
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Classification
- Olfactory receptors, family 1
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemethyltransferase 13, eEF1A lysine and N-terminal methyltransferase
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Synonyms gene
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Synonyms gene name
- KIAA0859
- methyltransferase like 13
- deafness (recessive, non-syndromic) modifier 1
- eEF1A lysine and N-terminal methyltransferase
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-11-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- 7BS protein lysine methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data