Mouse Monoclonal IgG Anti-AGE-4 Methylglyoxal modifiied [14B5]
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Catalog numberAGE4-010
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PricePlease ask
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Size10 µg
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AvailabilityPlease contact us to check the availability
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ImmunogenMethylglyoxal-modified BSA
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SpecificityAGE-4 (Methylglyoxal-modified proteins); in Western Blot no cross-reaction with glucose-modified AGEs (AGE-1), AGE-2 (Glycerolaldehyde-modified), AGE-3 (Glycolaldehyde-modified), AGE-5 (Glyoxal-modified), AGE-6 (3-DG-modified), CEL-BSA, CML-BSA and BSA
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Species reactivitynot relevant
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Product presentationProtein G affinity purified monoclonal antibody in PBS with 2% Block Ace as stabilizer and 0.1% Proclin as preservative
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Product categoryPrimary Antibody
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Antibody s raised inMouse
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Antibody s isotypeIgG
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Antibody s clone14B5
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Product is supplied asliquid concentrate
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Recommended concentration for use0,1 µg/ml (WB) and 0,05 µg/ml (ELISA)
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Protein concentration0.25 mg/ml
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Positive controlhuman lens, arteriosclerotic plaques
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Tested applicationsELISA, WB
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Recommended secondary reagentAs secondary system in ELISA and Western Blot we recommend biotinylated anti-mouse IgG antibody (Art. No. ZU102) in combination with streptavidin-HRPO conjugate (Art. No. ZU054) or streptavidin-alkaline phosphatase (Art. No. ZU051).
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Storage methodStore at -20°C
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UniProt numberGo to UniProt/SwissProt
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Litterature1. Takeuchi M. , Makita Z., Bucala R., Suzuki T., Koike T., and Kameda Y. (2000) Immunological evidence that non-carboxymethyllysine Advanced Glycation End-products are produced from short chain sugars and dicarbonyl compounds in vivo. Mol. Med. 6; 114-125. 2. Takeuchi M., Yanase Y., Matsuura N., Yamagishi S., Kameda Y., Bucala R., and Z. Matika (2001) Immunological detection of a novel Advanced Glycation End-Product. Mol. Med. 7; 783-791. 3. Van Herreweghe F. et al. (2002) Tumor necrosis factor-induced modulation of glyoxalase I activities through phosphorylation by PKA results in cell death and is accompanied by the formation of a specific methylglyoxal-derived AGE.: Proc .Natl. Acad. Sci. U S A.;99(2):949-954. 4. Speer O. et al.(2003) Rapid suppression of mitochondrial permeability transition by methylglyoxal. Role of reversible arginine modification. J. Biol .Chem. 278(37):34757-63.
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Tips*Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolMIR1302-4, PIRC18, PIRC16, SNORD114-4, IGKV2OR2-4, MIR941-4, PIRC19, PIRC17
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Short nameAGE
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TechniqueMouse monoclonal, anti-, Mouse, anti, IgG, antibody to, antibodies, IgGs, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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IsotypeIgG, IgG
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SpeciesMouse, Mouses
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Alternative nameAGE-4 Methylglyoxal modifiied
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Alternative techniquemurine, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namemicroRNA 1302-4
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1302 family
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 18
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 16
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 114-4
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Synonyms
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Locus
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Discovery year2006-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-4 (pseudogene)
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-4
- immunoglobulin kappa variable 2/OR2-4 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
Gene info
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Identity
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Gene
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Long gene namemicroRNA 941-4
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Synonyms gene
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Synonyms
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Locus
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Discovery year2007-10-24
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 19
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
Gene info
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Identity
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Gene
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Long gene namepiwi-interacting RNA cluster 17
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Locus
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Discovery year2009-11-05
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Entrez gene record
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Pubmed identfication
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Classification
- Piwi-interacting RNA clusters
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
