Mouse Monoclonal antibody to detect RPA70
Monoclonal Antibody (Clone#8C3-D12-H10)
Human, Mouse, Rat, Monkey
Recombinant human RPA70 protein fragments expressed in E.coli
RPA70 (Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, RPA1)
Affinity purified antibody is supplied in liquid form in PBS (pH 7.4) containing with 0.02% sodium azide and 50%
Antibody's positive controles
Western blot: Hela, 293T, C6, 3T3 and K562 cell lysates; Western blot: Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates; Immunoprecipitation: HeLa cell lysates; Immunofluorescence: HeLa cells
Approved applications with antibody dilution
Western blot; 1:1000, Immunofluorescence; 1:100, Immunoprecipitation
Replication protein A 70 kDa DNA-binding subunit plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Shipped to your lab under gel pack
Manifactured in USA. For Research Use Only! Not to be used in humans
This antibody is available to order since May 2017
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.