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Mouse Monoclonal Anti-RPA70 Antibody (8C3-D12-H10)

  • Catalog number
    A1317-100
  • Product name
    Mouse Monoclonal Anti-RPA70 Antibody (8C3-D12-H10)
  • Size
    100 µg
  • Price
    397.45
  • Supplier
    Biovision
  • Description
    Mouse Monoclonal antibody to detect RPA70
  • Raised in
    Mouse
  • Clonality
    Monoclonal Antibody (Clone#8C3-D12-H10)
  • Reacts with
    Human, Mouse, Rat, Monkey
  • Immunogen
    Recombinant human RPA70 protein fragments expressed in E.coli
  • Target
    RPA70 (Replication protein A 70 kDa DNA-binding subunit, RP-A p70, Replication factor A protein 1, RF-A protein 1, Single-stranded DNA-binding protein, RPA1)
  • Research area
    Epigenetics
  • Gene number
    6117
  • Protein number
    P27694
  • Physical state
    Colorless liquid
  • Package form
    Affinity purified antibody is supplied in liquid form in PBS (pH 7.4) containing with 0.02% sodium azide and 50%
  • Purity
    Affinity purified
  • Antibody's positive controles
    Western blot: Hela, 293T, C6, 3T3 and K562 cell lysates; Western blot: Hela, A431, MCF7, COS7, HT1080 and K562 cell lysates; Immunoprecipitation: HeLa cell lysates; Immunofluorescence: HeLa cells
  • Approved applications with antibody dilution
    Western blot; 1:1000, Immunofluorescence; 1:100, Immunoprecipitation
  • Scientific context
    Replication protein A 70 kDa DNA-binding subunit plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage.
  • Handling and storage
    Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
  • Delivery
    Shipped to your lab under gel pack
  • Precautions
    Manifactured in USA. For Research Use Only! Not to be used in humans
  • Released
    This antibody is available to order since May 2017
  • Additional description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse Monoclonal Anti-RPA70 Antibody (8C3-D12-H10)
  • Technique
    Mouse monoclonal, antibody to, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse monoclonal antibody to-RPA70 (Antibody to) (8C3-D12-H10)
  • Alternative technique
    antibodies
1. Gene info
MeSH Data
  • Name
  • Concept
    Scope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
Additional information
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