Mouse Monoclonal antibody to detect DsbA
Monoclonal Antibody (Clone#1A2-D4-G10)
Recombinant E.coli. DsbA protein fragments over expressed in E.coli
Thiol:disulfide interchange protein DsbA (Thiol:disulfide interchange protein DsbA, dsbA, dsf, ppfA)
Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol
Antibody's positive controles
Western blot: AKT1, AKT2, AKT3 and DSBA recombinant antigen
Approved applications with antibody dilution
Western blot; 1:1000
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Handling and storage
Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial
Shipped to your lab under gel pack
Manifactured in USA. For Research Use Only! Not to be used in humans
This antibody is available to order since May 2017
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.