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Affinity purified Mouse IgG2a Isotype Antibody FITC-Conjugated

  • Catalog number
  • Product name
    Affinity purified Mouse IgG2a Isotype Antibody FITC-Conjugated
  • Size
    50 mg
  • Price
  • Supplier
  • Clone
  • Source
  • Clonality
  • Category
    Isotype Control
  • Description
    The C1.18.4 immunoglobulin has unknown specificity. The isotype of this antibody is Mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
  • Reactivity
    Not specified / Inquire
  • Conjugation
  • Purification
    Affinity purified
  • Antibody's supplied with
    PBS pH 7.2, 0.09% NaN3, 0.2% BSA
  • Antibody's concentration
    0,5 mg/ml
  • Storage
    Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Antibody's is used for
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
  • Test
    A high affinity purification column was use to purify Mouse IgG2a Isotype Antibody FITC-Conjugated by Elabscience by chromatographic size exclusion. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Additional description
    1 of protein A or G purified by Elabscience will give more specificity than crude serum.
  • Properties
    If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
  • Additional conjugation
    Anti-FITC Antibody
  • Additional isotype
  • Group
  • Latin name
    Mus musculus
  • French translation
  • Gene target
  • Short name
    Mouse IgG2a Isotype Antibody FITC-Conjugated
  • Technique
    affinity, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, Isotypes and controls, mouses, purifications
  • Host
  • Label
  • Species
    Mouse, Mouses
  • Alternative name
    protein enriched Mouse IgG2a isoform (Antibody to) fluorecein-coupled
  • Alternative technique
MeSH Data
  • Name
  • Concept
    Scope note:Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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