-
Clone
ESEE122
-
Immunogen
Recombinant fragment corresponding to amino acids 329-530
-
Antibody s full description
Mouse Anti-Mouse HIF 1 alpha Monoclonal IgG1 Antibody, Clone: ESEE122: PerCP
-
Antibody s category
Monoclonal Antibodies
-
Antibody s other name
ARNT interacting protein Antibody, HIF1A Antibody, Hypoxia inducible factor 1 alpha Antibody, MOP1 Antibody, PASD8 Antibody
-
Raised in
Mouse
-
Antibody s target
HIF 1 alpha
-
Primary research fields
Cancer, Oxidative Stress, Cell Signaling, Epigenetics
-
Brandname
None
-
Antibodies applications
WB, IHC, ICC/IF, ELISA
-
Antibody s reactivity
Human, Mouse, Rat, Bovine
-
Antibody s dilutions
WB (1:1000), IHC (1:100), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
-
Purity
Protein G Purified
-
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
-
Antibody s concentration
1 mg/ml
-
Antibody s specificity
Detects ~116kDa. Specific for HIF1Alpha.
-
Storage recommendations
-20ºC
-
Shipping recommendations
Blue Ice or 4ºC
-
Antibody certificate of analysis
1 µg/ml of SMC-184 was sufficient for detection of HIF1α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
-
Antibody in cell
Cytoplasm , Nucleus
-
Tissue specificity
See included datasheet or contact our support service
-
Scientific context
Hypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response of hypoxia (1). The HIF1 complex consists of two subunits, HIF1-Alpha and HIF1-Beta, which are basic helix-loop-helix proteins of the PAS family (2). HIF1 regulates the transcription of a broad range of genes that facilitate responses to the hypoxic environment, including genes regulating angiogenesis, erythropoiesis, cell cycle, metabolism and apoptosis. The widely expressed HIF-1α is typically degraded rapidly in normoxic cells by the ubiquitin/proteasomal pathway. Under normoxic conditions, HIF-1α is proline hydroxylated leading to a conformational change that promotes binding to the von Hippel Lindau protein (VLH) E3 ligase complex; ubiquitination and proteasomal degradation follows (3, 4). Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway (5-7).
-
Bibliography
1. Sharp F.R. and Bernaudin M. (2004) Nat Rev Neurosci 5: 437-48. 2. Wang G.L., et al. (1995) Proc Natl Acad Sci U S A 92: 5510-4. 3. Jaakkola P., et al. (2001) Science 292: 468-72. 4. Maxwell P.H., et al. (1999) Nature 399: 271-5. 5. Fukuda R., et al. (2002) J Biol Chem 277: 38205-11. 6. Jiang B.H., et al. (2001) Cell Growth Differ 12: 363-9. 7. Laughner E., et al. (2001) Mol Cell Biol 21: 3995-4004.
-
Released date
1-Sep-2010
-
PubMed number
23549681
-
Tested applications
Immunocytochemistry/Immunofluorescence
-
Tested reactivity
Human
-
NCBI number
NP_034561.2
-
Gene number
15251
-
Protein number
Q61221
-
Antibody s datasheet
Contact our support service
-
Representative figure link
-
Representative figure legend
Immunohistochemistry analysis using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (SMC-184). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (SMC-184) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Membranous and cytoplasmic localization in the epidermis, positive hair follicles, muscle and dermis. . Western Blot analysis of Human HeLa cell lysates showing detection of HIF1 alpha protein using Mouse Anti-HIF1 alpha Monoclonal Antibody, Clone ESEE122 (SMC-184). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HIF1 alpha Monoclonal Antibody (SMC-184) at 1:500 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Mouse Anti-HIF1 alpha Antibody [ESEE122] used in Immunohistochemistry (IHC) on Mouse backskin (SMC-184) Mouse Anti-HIF1 alpha Antibody [ESEE122] used in Western Blot (WB) on Human HeLa cell lysates (SMC-184)
-
Warning information
Non-hazardous
-
Country of production
Canada
-
Total weight kg
1.4
-
Net weight g
0.1
-
Stock availability
In Stock
-
-
Description
The Mouse Anti-Mouse HIF1 alpha Monoclonal IgG1, Clone ESEE122 is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus