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Mouse anti CD4 antibody conjugated to FITC / CD8 antibody conjugated to PE / CD3 antibody conjugated to Cy5-PE

  • Catalog number
    X1178s
  • Product name
    Mouse anti CD4 antibody conjugated to FITC / CD8 antibody conjugated to PE / CD3 antibody conjugated to Cy5-PE
  • Size
    50 Tests
  • Price
    356.3
  • Supplier
    nordc
  • Category
    Primary Antibodies
  • Long description
    The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. CD3 indentifies human T cells expressing the 22-28,000 M.W. surface antigen. CD4 identifies human helper/inducer T cells expressing the 60,000 M. W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. Identification of CD8 on human cytotoxic/ suppressor T cells expressing the 32 and 43,000 M.W. surface antigens. Can be used for any of the following:_x000B__x000B_Monitoring of T cells subsets in peripheral blood._x000B_Analysis of CD3 complex related to the T cell antigen receptor._x000B_Analysis of T cell subsets involved in helper/inducer functions._x000B_Characterization of subtypes of T cell leukemia's and lymphomas._x000B_Studies of AIDS/HIV virus infection._x000B_Analysis of NK subsets._x000B_Study of cell mediated cytotoxicity.
  • Antibody come from
    CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes. CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
  • Other description
    Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Affinity Chromatography
  • Clone
    7E14/17D8/M2AB
  • Antigen-antibody binding interaction
    Mouse anti CD4 antibody conjugated to FITC / CD8 antibody conjugated to PE / CD3 antibody conjugated to Cy5-PE Antibody
  • Antibody is raised in
    Mouse
  • Antibody's reacts with
    Human
  • Antibody's reacts with these species
    This antibody doesn't cross react with other species
  • Antibody's specificity
    No Data Available
  • Application
    Flow Cytometry
  • Antibody's suited for
    Fluorescence Information:_x000B_ Dye Excitation Emission_x000B_ FITC 488 nm 530 EM max nm_x000B_ RPE 488 nm 565 EM max nm _x000B_ RPE-Cy-5 488 nm 670 EM max nm
  • Storage
    4ºC
  • Relevant references
    1. Orfao A, Gonz‡lez de Buitrago JM La citometr’a de flujo en el laboratorio cl’nico. Sociedad espa–ola de bioqu’mica cl’nica y patolog’a Molecular 1995._x000B_2. Stetler-Stevenson M. Flow cytometry analysis of lymphomas and lymphoproliferative disorders. Semin Hematol 2001 Apr;38(2):111-23._x000B_3. MenŽndez P, et al. Comparison between a lyse-and-then-wash method and a lyse-non-wash technique for the enumeration of CD34+ hematopoietic progenitor cells. Cytometry (Comm. Clin. Cytometry) 34: 264-271 (1998)_x000B_4. GratamaJW, MenŽndez P, Kraan J, Orfao A. Loss of CD34+ hematopoietic progenitor cells due to washing can be reduced by the use of fixative-free erytrocyte lysing reagents. J Immunol. Methods 239: 13-23 (2000)_x000B_5. Protection of Laboratory Workers from occupationally acquired infections. Second edition; approved guideline (2001). Villanova PA: National Committee for Clinical Laboratory Standards; Document M29-A2._x000B_6. Procedures for the collection of diagnostic blood specimens by venipuncture- approved standard; Fifth edition (2003). Wayne PA: National Committee for Clinical Laboratory Standards; Document H3-A5._x000B_7. Clinical applications of flow cytometry: Quality assurance and immunophenotyping of lymphocytes; approved guideline (1998). Wayne PA: National Committee for Clinical Laboratory Standards; Document H42-A._x000B_8. Lima M et al. TCR +/CD4+ Large Granular Lymphocytosis. A new Clonal T-Cell Lymphoprolipherative Disorder. American Journal of Pathology, 163 (2):763-771 (2003)_x000B_9. Gorczyza W. et al. An approach to diagnosis of T-cell lymphoprolipherative disorders by flow cytometry. Cytometry (Clinical cytometry) 50:177-190 (2002)_x000B_10. Braylan RC, Orfao A, Borowitz MJ, Davis BH. Optimal number of reagents required to evaluate hematolymphoid neoplasias: results of an international consensus meeting. Cytometry 46: 23-7 (2001)_x000B_11. Jennings CD, Foon KA. Recent advances in flow cytometry: application to the diagnosis of hematologic malignancy. Blood 90(8): 2863-2892 (1997)_x000B_12. Reichert et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol 60:190-208 (1991) _x000B_13. Prince HK et al. Influence of racial background on the distribution of T-cell subsets and Leu-11 positive lymphocytes in healthy_x000B_blood donors. Diagn Immunol. 3: 33-39 (1985) _x000B_14. Kotylo PK et al. Reference ranges for lymphocyte subsets in pediatric patients. Am J Clin Pathol 100:111-5 (1993)
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones. Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
  • Properties
    If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
  • Conjugation
    Cy5.5, Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
  • Short name
    Mouse anti CD4 antibody conjugated FITC / CD8 antibody conjugated PE / CD3 antibody conjugated -PE
  • Technique
    Antibody, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
  • Host
    mouse
  • Label
    FITC
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to CD4 molecule (Antibody to) coupled to fluorecein / CD8 (Antibody to) coupled to peroxidase / CD3 (Antibody to) coupled to cyanine 5-peroxidase
  • Alternative technique
    antibodies
  • Alternative to gene target
    CD4 molecule, CD4mut, CD4 and IDBG-15034 and ENSG00000010610 and 920, this GO :0001816 and cytokine production and biological process this GO :0001948 and glycoprotein binding and molecular function this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005201 and extracellular matrix structural constituent and molecular function this GO :0005515 and protein binding and molecular function this GO :0005769 and early endosome and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006948 and induction by virus of host cell-cell fusion and biological process this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007167 and enzyme linked receptor protein signaling pathway and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0015026 and coreceptor activity and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030217 and T cell differentiation and biological process this GO :0030260 and entry into host cell and biological process this GO :0031295 and T cell costimulation and biological process this GO :0032507 and maintenance of protein location in cell and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042289 and MHC class II protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0045058 and T cell selection and biological process this GO :0045086 and positive regulation of interleukin-2 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045234 and protein palmitoleylation and biological process this GO :0045860 and positive regulation of protein kinase activity and biological process this GO :0050690 and regulation of defense response to virus by virus and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0050863 and regulation of T cell activation and biological process this GO :0050870 and positive regulation of T cell activation and biological process, protein homodimerization activity, this GO :0001948: glycoprotein binding and also this GO :0004872: receptor activity and also this GO :0004888: transmembrane signaling receptor activity and also this GO :0005201: extracellular matrix structural constituent and also this GO :0005515: protein binding and also this GO :0008270: zinc ion binding and also this GO :0015026: coreceptor activity and also this GO :0019899: enzyme binding and also this GO :0019901: protein kinase binding and also this GO :0042289: MHC class II protein binding and also this GO :0042803: protein homodimerization activity, this GO :0001948: glycoprotein binding, this GO :0004872: receptor activity, this GO :0004888: transmembrane signaling receptor activity, this GO :0005201: extracellular matrix structural constituent, this GO :0005515: protein binding, this GO :0008270: zinc ion binding, this GO :0015026: coreceptor activity, this GO :0019899: enzyme binding, this GO :0019901: protein kinase binding, this GO :0042289: MHC class II protein binding, this GO :0042803: protein homodimerization activity, Cell surfaces, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098
MeSH Data
  • Name
  • Concept
    Scope note:Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
  • Tree numbers
    • E01.370.225.500.363.342
    • E01.370.225.500.386.350
    • E05.196.712.516.600.240.350
    • E05.200.500.363.342
    • E05.200.500.386.350
    • E05.242.363.342
    • E05.242.386.350
  • Qualifiers
    classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
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