Mouse anti CD3 antibody conjugated to FITC - CD38 antibody conjugated to PE

• Catalog number
  0338
• Price
  Please ask
• Size
  100 Tests

• Category
  Primary Antibodies
• Long description
  The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is expressed on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M. W. surface antigen. CD38 antigen is an integral membrane glycoprotein, M.W. 45 kD. The CD38 antigen is present on approximately all pre-B lymphocytes, plasma cells and thymocytes. CD38 antigen is also expressed on activated T Lymphocytes, NK Cells, monocytes, myeloblasts and erythroblasts. CD38 is co-expressed on CD34+ cells, but is not expressed on pluripotent stem cells. CD38 antigen is present on T and B lymphoblastic leukemia (ALL), Burkitts' lymphoma, multiple myeloma and acute myeloid leukemia (AML).
• Antibody come from
  CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes.
• Other description
  Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
• Clone
  not specified
• Antigen antibody binding interaction
  Mouse anti CD3 antibody conjugated to FITC - CD38 antibody conjugated to PE Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  CD Marker
• Application
  Flow Cytometry
• Antibody s suited for
  PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20¼C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  4ºC
• Relevant references
  1). Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293. _x000B__x000B_2). T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9. _x000B__x000B_3). Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21. _x000B__x000B_4). Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23. _x000B__x000B_5) CD38 Molecule: Structural and Biochemical Analysis on Human T Lymphocytes, Thymocytes and Plasma Cells. J. Immunol, 1990 Aug; 145(3):878-84. Alessio, M. Roggero, S.; Funaro, A. ; De Monte L.B.,: Peruzzi, L.; Geuna, M.; Malavasi,F. _x000B__x000B_6) Differences Amoung Mononuclear Cell Subpopulations in HIV Seropositive or Seronegative Homosexual and Heterosexual men as Determined by Four Color Flow Cytometry. J. Acquir Immume Defic Syndr., 1990;3(8):747-56. Schnizlein-Bick, C.T.; Magier, M.R.; Jones, R.B.; Fife, K.H.; Katz, B., P.; Walker, E.B. _x000B__x000B_7) Quantitative Comparison of Myeloid Antigens on Five Lineages of Mature Peripheral Blood Cells. J. Leukoc Bio. 1990, Au;48(2):138-48. Terstappen, L.W.; Hollander, Z.; Meiners, H.; Loken, M.R. _x000B__x000B_8) B-cell Surface Phenotypes of Proliferating Myeloma Cells: Target Antigens for Immunotherapy. Am. J. Hematol. 1990, Fe;33(2):101-9, Chan, C.S; Wormsley, S.B.; Pierce, L. E.; Peter, J.B.; Schechter, G.P. _x000B__x000B_9) Interrelationships Between Serologic Markers of Immune Activation and T Lymphocyte Subsets in HIV Infection. J. Acquir Immune Defic Syndr. 1990;3(5):525-30. Prince, H.E.; Kleinman, S. Czaplicki, C.; John, J.; Williams, A.E. _x000B__x000B_10) Sequential Gererations of Hematopoietic Colonies Derived from Single Nonlineage-committed CD34+ CD38- Progenitor Cells. Blood 1991, Mar 1;77(6):1218-27. Terstappen, L.W.; huang, S.; Safford, M. Lansdorp, P.M.; Loken, M.R. _x000B__x000B_11) Involvement of Multilineage CD38 Molecule in a Unique Pathway of Cell Activation and Proliferation. J. Immunol, 1990 Oct 1;145(8):2390-6Funaro, A.; Spagnoli, G.C.; Ausiello, C.M.; Alessio, M.; Roggero, S.; Delia, D.; Zaccolo, M.; Malavasi, F. _x000B__x000B_12) Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus 6. Nat.349(6309): 533-5, 1991. Lusso, P.; De Maria, A.; Malnati, M.; Lori, F.; DeRocco, S.E.;Baseler, M.; Gallo, R.C. _x000B__x000B_13) Evolutionary Conservation of Surface Molecules That Distinguish T Lymhocyte Helper/Inducer and T Cytotoxic/Suppressor Subpopulations in Mouse and Man. J. Exp Med 153,310, 1981. Ledbetter, J. A.; Evans, R.L.; Lipiniski, M.; Cunningham-Rundles, C.; Good, R.A; Herzenberg, L. A. _x000B__x000B_14) Circulating Antigen-Specific Suppressor T Cells in a Healthy Woman: Mechanism of Action and Isolation with a Monoclonal Antibody. Clin. Res. 29, 365a, 1981. Engleman, E. G.; Benike, C. J.; Evans, R.L _x000B__x000B_15) Induction of Immunoglobulin Secreting Cells in the Allogeneic Mixed Leukocyte Reaction: Regulation by Helper and Suppressor Lymphocyte Subsets in Man. J. Imm.127,931, 1981. Kotzin, B.L.; Benike, C.J.; Engleman, E. G.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  CD3   conjugated   CD38   conjugated  
• Gene symbol
  CD38
• Short name
  Mouse anti CD3 antibody conjugated FITC - CD38 antibody conjugated PE
• Technique
  Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
• Host
  mouse
• Isotype
  IgG1 (F)/IgG1 (PE)
• Label
  FITC and PE
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD3 (antibody to-) coupled to fluorecein - CD38 molecule (antibody to-) coupled to peroxidase
• Alternative technique
  antibodies, murine, fluorescine
• Alternative to gene target
  CD38 molecule, ADPRC 1 and T10, CD38 and IDBG-10668 and ENSG00000004468 and 952, hydrolase activity, nuclei, Cd38 and IDBG-161475 and ENSMUSG00000029084 and 12494, CD38 and IDBG-643982 and ENSBTAG00000013569 and 327677

Gene info

• Identity
  HGNC:1667
• Gene
  CD38
• Long gene name
  CD38 molecule
• Synonyms gene name
  • CD38 antigen (p45)
• Synonyms name
  • ADP-ribosyl cyclase 1
  • NAD(+) nucleosidase
• GenBank acession
  • D84276
• Locus
  4p15.32
• Discovery year
  1986-01-01
• Entrez gene record
  952
• Pubmed identfication
  • 9074508
  • 2319135
• RefSeq identity
  • NM_001775
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000048206

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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