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Category
Primary Antibodies
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Long description
Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The FITC conjugate is particularly useful in these applications as a secondary antibody is not necessary for visualization. Also, a specific anti-FITC antibody conjugate can be used for signal amplification if desired.
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Antibody come from
GST-fused recombinant protein derived from the C terminal of the p73 protein.
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
MoBU-1
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Antigen antibody binding interaction
Mouse anti BrdU (bromodeoxyuridine) antibody conjugated to FITC Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
BrdU Incorporated Cells
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry, Flow Cytometry
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Antibody s suited for
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Zeng, X., et al. 'p300 does not require its acetylase activity to stimulate p73 function.' J. Biol. Chem. 2001: 276, 48-52._x000B__x000B_2. Dobbelstein M, Roth J: The large T antigen of simian virus 40 binds and inactivates p53 but not p73. J Gen Virol 1998;79:3079-3083._x000B__x000B_3. Higashino F, et al: Adenovirus e4orf6 oncoprotein modulates the function of the p53-related protein, p73. Proc Natl Acad Sci U S A 1998 Dec 22;95(26):15683-15687._x000B__x000B_4. Lo Cunsolo C, et al: Refined chromosomal localization of the putative tumor suppressor gene TP73. Cytogenet Cell Genet 1998;82(3-4):199-201._x000B__x000B_5. Kovalev S, et al: Expression level, allelic origin, and mutation analysis of the p73 gene in neuroblastoma tumors and cell lines. Cell Growth Differ 1998 Nov;9(11):897-903._x000B__x000B_6. Zhu J, et al: The potential tumor suppressor p73 differentially regulates cellular p53 target genes. Cancer Res 1998 Nov 15;58(22):5061-5065._x000B__x000B_7. De Laurenzi V, et al: Two new p73 splice variants, gamma and delta, with different transcriptional activity. J Exp Med 1998 Nov 2;188(9):1763-1768._x000B__x000B_8. Nimura Y, et al: p73, a gene related to p53, is not mutated in esophageal carcinomas. Int J Cancer 1998 Nov 9;78(4):437-440._x000B__x000B_9. Marin MC et. al. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Molecular and Cellular Biology, 1998;18(11):6316-6324.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
