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Category
Primary Antibodies
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Long description
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
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Antibody come from
Synthetic peptide derived from the human NOL4 protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
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Clone
HIS.H8/EH158
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Antigen antibody binding interaction
Mouse anti 6x His Monoclonal Antibody Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
6x His tagged proteins
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
WB, Immunostaining,
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Antibody s suited for
Antibody can be used for Western blotting (1:400 dilution) and immunohistochemistry on paraffin embedded tissues (2 µg/ml). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Ueki, N., et al. 'NOLP: identification of a novel human nucleolar protein and determination of sequence requirements for its nucleolar localization.' Biochem Biophys Res Commun 1998;252:97-102._x000B__x000B_2. Wang, S.S., et al. 'Identification of novel methylation markers in cervical cancer using restriction landmark genomic scanning.' Cancer Res 2008;68:2489-2497._x000B__x000B_3. Visintin R, et al. 'The nucleolus: the magician's hat for cell cycle tricks.' Curr Opin Cell Biol 2000;12:372Ð7._x000B__x000B_4. Olson MO, et al. 'The nucleolus: an old factory with unexpected capabilities.' Trends Cell Biol 2000;10:189Ð96.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
histidine
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
