-
Also known as
Rat anti Mouse kappa light chain (biotin)
-
Other names
N/A
-
Category
Antibodies
-
Subcategory
Mnoclonal antibodies
-
Gene name
LOC100037688; N/A; N/A; N/A
-
Gene name synonims
LOC100037688; N/A; N/A; N/A
-
Other gene names
LOC100037688; N/A; N/A; N/A
-
Clonality
Monoclonal
-
Immunoglobulin isotype
IgG2a kappa
-
Clone
LO-MK-1
-
Host organism
Rat (Rattus norvegicus)
-
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
-
Specificity and cross reactivity
Murine; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
-
Purification method
N/A
-
Form Appearance
PBS with 0.1% NaN3 and 50% glycerol.
-
Concentration
1.0 mg/ml
-
Storage and shipping
Keep the antibody after aliquoting at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies, thus reducing their activity. It is recommended to minimize such cycles as much as possible.
-
Tested applications
ELISA (EIA), Immunohistochemistry (IHC)
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
-
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus, Rattus norvegicus
-
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
French translation
anticorps
