RAT ANTI MOUSE F4/80:Low Endotoxin Antibody

  • Catalog number
    GEN216412
  • Price
    Please ask
  • Size
    500ug
  • Also known as
    F4/80
  • Other names
    EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71; EGF-like module receptor 1; cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EMR1 hormone receptor
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    F4/80
  • Gene name synonims
    N/A
  • Other gene names
    Emr1; Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7; Gpf480
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2b
  • Clone
    Cl:A3-1
  • Host organism
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    F4/80 The F4/80 antibody recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. Clone Cl:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Low Endotoxin (Purified IgG - liquid)
  • Concentration
    IgG concentration 1 miligram per 1 mililiter
  • Storage and shipping
    the antibody should be stored at -20 degrees Celsius. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS), Immunoprecipitation (IP), RadioImmunoassay (RIA), Immunohistology - Resin (IHC), Western Blot (WB), Immunohistology - Frozen (IHC), Immunohistology - Paraffin (IHC), Functional Assay (FA)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • French translation
    anticorps
  • Gene target
  • Short name
    ANTI MOUSE F4/80: Endotoxin Antibody
  • Technique
    anti mouse, Antibody, Mouse, anti, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, mouses
  • Host
    Rat
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-RAT ANTI MOUSE F4/80:Low Endotoxin
  • Alternative technique
    antibodies, murine
MeSH Data
  • Name
  • Concept
    Scope note: Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.
  • Tree numbers
    • E01.370.225.124.470
    • E01.370.225.875.150.570
    • E05.091.570
    • E05.200.124.470
    • E05.200.875.150.570
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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