RAT ANTI MOUSE CD34:FITC Antibody

  • Catalog number
    GEN215258
  • Price
    Please ask
  • Size
    0.025 miligrams
  • Also known as
    CD34
  • Other names
    hematopoietic progenitor cell antigen CD34 isoform 2; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; cluster designation 34; CD34 antigen; N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    CD34
  • Gene name synonims
    N/A
  • Other gene names
    Cd34; Cd34; AU040960; N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a
  • Clone
    MEC14.7
  • Host organism
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    CD34 This item recognises the murine CD34 cell surface antigen, which is expressed by endothelial cells and by haematopoietic stem cells. This antibody recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Clone MEC14.7 appears to recognise a subset of the stem cell population recognised by clone RAM34, and it is thought that this is due to differences in the epitope recognised by the two antibodies.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
  • Concentration
    IgG concentration 0.1 miligram per 1 mililiter
  • Storage and shipping
    Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • French translation
    anticorps
  • Gene target
    CD34  
  • Gene symbol
    CD34
  • Short name
    ANTI MOUSE CD34:FITC Antibody
  • Technique
    anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
  • Host
    Rat
  • Label
    FITC
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-RAT ANTI MOUSE CD34:FITC
  • Alternative technique
    antibodies, murine, fluorescine
  • Alternative to gene target
    CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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