RAT ANTI MOUSE CD335:FITC Antibody

  • Catalog number
    GEN224646
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    CD335
  • Other names
    natural cytotoxicity triggering receptor 1; Natural cytotoxicity triggering receptor 1; natural cytotoxicity triggering receptor 1; mAR-1; NK-p46; mNKp46; activating receptor 1; lymphocyte antigen 94; MAR1 (mouse activating receptor 1); natural killer cell p46-related protein; natural cytotoxicity triggering receptor 1; Activating receptor 1; mAR-1; Lymphocyte antigen 94; Natural killer cell p46-related protein
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    CD335
  • Gene name synonims
    N/A
  • Other gene names
    Ncr1; Ncr1; Ly94; NKp46; Ly94; mAR-1; NK-p46; NKp46; mNKp46
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a
  • Clone
    29A1.4
  • Host organism
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    CD335 Clone 29A1.4 recognises mouse CD335, uniquely expressed by resting and activated natural killer (NK) cells. It is a major NK cell lysis receptor for autologous pathogen-infected and tumour target cells during natural cytotoxicity responses. Ligands of CD335 include viral haemagglutinins (HAs) and heparan sulphate proteoglycans (HSPGs) on the surface of tumour cells. _x000D_Staining with clone 29A1.4 is not strain specific and the antibody has been used to stain C57BL/6, SJL, CBA/CA and BALB/c strains. This clone also activates NK cells in vitro. It does not deplete NK cells in vivo.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
  • Concentration
    IgG concentration 500ug/1000ul
  • Storage and shipping
    Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 12 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • French translation
    anticorps
  • Gene target
    CD335  
  • Gene symbol
    NCR1
  • Short name
    ANTI MOUSE CD335:FITC Antibody
  • Technique
    anti mouse, Antibody, Mouse, anti, FITC, antibody to, anti mouse antibodies, antibodies against human proteins, antibodies for, Fluorescein, mouses
  • Host
    Rat
  • Label
    FITC
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-RAT ANTI MOUSE CD335:FITC
  • Alternative technique
    antibodies, murine, fluorescine
Gene info
  • Identity
  • Gene
  • Long gene name
    natural cytotoxicity triggering receptor 1
  • Synonyms gene
  • Synonyms gene name
    • lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-04-09
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Immunoglobulin like domain containing
    • CD molecules
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee