Uhrf1 Antibody
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Catalog numberF53188-0.4ML
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PricePlease ask
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenUhrf1
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityMouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, ELISA
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Recommended dilutionsWestern blot: 1:1000-2000
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NotesTitration of the Uhrf1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Uhrf1 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ8VDF2
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PurityAntigen affinity
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DescriptionMultidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
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ImmunogenThis mouse Uhrf1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from the C-terminal region of mouse Uhrf1.
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StorageAliquot the Uhrf1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolUHRF1BP1L, UHRF1BP1, UHRF1
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Short nameAnti-Uhrf1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Uhrf1
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameUHRF1 binding protein 1 like
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Synonyms
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Locus
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Discovery year2007-11-27
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameUHRF1 binding protein 1
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Synonyms gene
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Synonyms gene name
- chromosome 6 open reading frame 107
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-25
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameubiquitin like with PHD and ring finger domains 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-03-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Ring finger proteins
- PHD finger proteins
- Tudor domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data