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Long name
SPANXB1 Polyclonal Antibody, Biotin Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
Biotin
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
SPANXB1
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Specificity
This is a highly specific antibody against SPANXB1.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Source
KLH conjugated synthetic peptide derived from human SPANXB1
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Tested applications
WB, IHC-P
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Recommended dilutions
WB(1:100-1000), IHC-P(1:100-500)
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Crossreactivity
Human
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
A variety of morphological and molecular changes are required for mature spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPANX (sperm protein associated with the nucleus, X-linked) family members are sperm- and testis-specific proteins containing between 97-103 amino acids, whose genes form a cluster on chromosome X. Sharing a high level of sequence similarity, SPANX-A, -B, -C, -D and -E localize to both cytoplasm and nucleus where they are associated with nuclear craters. SPANX-B (Sperm protein associated with the nucleus on the X chromosome B/F) is a 103 amino acid protein that is detected in round and elongating spermatids.
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
N/A
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Synonyms
Cancer/testis antigen 11.2; Cancer/testis antigen family 11, member 2; CT11.2; hCG2036708; Nuclear associated protein SPAN Xb; Nuclear-associated protein SPAN-Xb; Nuclear-associated protein SPAN-Xf; SPAN Xb protein; SPAN Xf; SPANX; SPANX B; SPANX C; SPANX F; SPANX family member B/F; SPANX family, member B1; SPANX family, member B2; SPANX family, member F1; SPANX-B; SPANX-F; SPANXB; SPANXB2; SPANXF; SPANXF1; Sperm protein associated with the nucleus on the X chromosome B/F; Sperm protein associated with the nucleus, X chromosome, family; Sperm protein associated with the nucleus, X chromosome, family member B1; Sperm protein associated with the nucleus, X chromosome, family member B2; SPNXB_HUMAN.
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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French translation
anticorps
