Smac Antibody / Diablo

• Catalog number
  R32332
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Smac / Diablo
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ELISA
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the Smac antibody should be determined by the researcher.
• Intented use
  This Smac antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q9NR28
• Purity
  Antigen affinity
• Description
  Diablo homolog (DIABLO), also referred to as second mitochondria-derived activator of caspases or SMAC, is a mitochondrial protein that in humans is encoded by the DIABLO (direct IAP binding protein with low pI) gene on chromosome 12. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Immunogen
  Amino acids 56-239 of human Smac/Diablo were used as the immunogen for the Smac antibody.
• Storage
  After reconstitution, the Smac antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Smac   Diablo  
• Gene symbol
  DIABLO
• Short name
  Anti-Smac / Diablo
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Smac / Diablo
• Alternative technique
  antibodies
• Alternative to gene target
  diablo, IAP-binding mitochondrial protein, DIABLO and IDBG-62263 and ENSG00000184047 and 56616, protein binding, Plasma membranes, Diablo and IDBG-199693 and ENSMUSG00000029433 and 66593, DIABLO and IDBG-632769 and ENSBTAG00000004199 and 493999

Gene info

• Identity
  HGNC:21528
• Gene
  DIABLO
• Long gene name
  diablo IAP-binding mitochondrial protein
• Synonyms gene name
  • diablo, IAP-binding mitochondrial protein
• Synonyms
  • SMAC
  • DIABLO-S
  • FLJ25049
  • FLJ10537
  • DFNA64
• Synonyms name
  • second mitochondria-derived activator of caspase
• GenBank acession
  • AF262240
• Locus
  12q24.31
• Discovery year
  2003-10-27
• Entrez gene record
  56616
• Pubmed identfication
  • 12749848
  • 17237824
  • 21722859
• RefSeq identity
  • NM_019887
• VEGA ID
  OTTHUMG00000157014

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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