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Category
Primary Antibodies
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Long description
SPARC is a key factor in cell-matrix interactions and possibly tumour aggressiveness. The SPARC gene, which encodes a multifunctional glycoprotein with roles in tissue development, remodelling and fibrosis. A regulator of cell-extracellular matrix (ECM) interactions, SPARC represents a major factor in the ECM remodelling occurring during tumour invasion. in silico analysis reveals 4 UTR-SNPs located in the 3 -UTR of the SPARC gene, corresponding to 1474 g a, 1551 g c, 1922 t g and 2072 c t changes, which are significantly associated with tumoral state of the tissue. Of all hits, the 2072 SPARC polymorphism had the best association with cancer._x000B__x000B_SPARC therefore is a gene involved in a number of diseases including rheumatoid arthritis, scleroderma, tumor development and metastasis. SPARC variants have been detected in tumour samples of patients with acute myeloblastic leukemia (AML).
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Antibody come from
Synthetic peptide derived from a mutant form of the human PINK1 protein
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti SPARC antibody conjugated to Biotin Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human SPARC, may cross react with SPARCL1
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Immunohistochemistry
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Antibody s suited for
Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Matsuda, N., et al. ÔPINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy.Õ J. Cell. Biol., 189, 211-221 (2010)_x000B__x000B_2. Vives-Bauza, C., et al. ÔPINK1-dependent recruitment of Parkin to mitochondria in mitophagy.Õ Proc. Natl. Acad. Sci. USA, 107, 378-383 (2010)_x000B__x000B_3. Valente, E.M., et al. ÔPINK1 mutations are associated with sporadic early-onset parkinsonism.Õ Ann. Neurol., 56, 336-341 (2004)_x000B__x000B_4. Geisler, S., et al. ÔThe PINK1/Parkin-mediated mitophagy is compromised by PD-associated mutations.Õ Autophagy, 6, 871-878 (2010)_x000B__x000B_5. Valente, E.M., et al. ÔHereditary early-onset Parkinson's disease caused by mutations in PINK1.Õ Science, 304, 1158-1160 (2004)_x000B__x000B_6. Silvestri, L., et al. ÔMitochondrial import and enzymatic activity of PINK1 mutants associated to recessive parkinsonism.Õ Hum. Mol. Genet., 14, 3477-3492 (2005)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
CLMAT3, SPARC
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Short name
Rabbit anti SPARC antibody conjugated
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Technique
Antibody, Rabbit, anti, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates
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Host
Rabbit, Rabbits
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Isotype
not specified
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Label
Biotin
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Alternative name
production species: rabbit antibody to secreted protein, acidic, cysteine-rich (osteonectin) (antibody to-) coupled to biotinilated
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Alternative technique
antibodies, rabbit-anti
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Alternative to gene target
secreted protein, acidic, cysteine-rich (osteonectin), ON, SPARC and IDBG-54562 and ENSG00000113140 and 6678, extracellular matrix binding, nuclei, Sparc and IDBG-175174 and ENSMUSG00000018593 and 20692, SPARC and IDBG-646341 and ENSBTAG00000014835 and 282077
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