Paxillin Antibody, FITC Conjugated

  • Catalog number
    bs-3539R-FITC
  • Price
    Please ask
  • Size
    0.1ml
  • Long name
    Paxillin Polyclonal Antibody, FITC Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    FITC
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    Paxillin
  • Specificity
    This is a highly specific antibody against Paxillin.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic peptide derived from human Paxillin
  • Gene ID number
    5829
  • Tested applications
    IF(IHC-P)
  • Recommended dilutions
    IF(IHC-P)(1:50-200)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    494nm/518nm
  • Synonyms
    Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; FLJ16691; PAXI_HUMAN; Paxillin; PXN protein.
  • Properties
    If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
  • Additional conjugation
    Anti-FITC Antibody
  • French translation
    anticorps
  • Gene target
  • Short name
    Anti-Paxillin
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    FITC
  • Alternative name
    Anti-Paxillin PAb FITC
  • Alternative technique
    antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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