NIPP1/ARD1 Antibody, FITC Conjugated
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Catalog numberbs-3658R-FITC
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PricePlease ask
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Size0.1ml
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Long nameNIPP1/ARD1 Polyclonal Antibody, FITC Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationFITC
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenNIPP1/ARD1
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SpecificityThis is a highly specific antibody against NIPP1/ARD1.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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Subcellular locationNucleus
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SourceKLH conjugated synthetic peptide derived from human NIPP1
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Gene ID number5511
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundInhibitor subunit of the major nuclear protein phosphatase 1 (PP1). It has RNA binding activity but does not cleave RNA and may target PP1 to RNA associated substrates. May also be involved in pre mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission494nm/518nm
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SynonymsActivator of RNA decay; ARD 1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP1; Nuclear inhibitor of protein phosphatase 1; Nuclear subunit of PP1; PPP1R8; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RNase E.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolPPP1R8, TRIM23, NAA10
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Short nameAnti-NIPP1/ARD1
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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IsotypeImmunoglobulin G (IgG)
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LabelFITC
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Alternative nameAnti-NIPP1/ARD1 PAb FITC
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameprotein phosphatase 1 regulatory subunit 8
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Synonyms gene name
- protein phosphatase 1, regulatory (inhibitor) subunit 8
- protein phosphatase 1, regulatory subunit 8
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
- Small nucleolar RNA protein coding host genes
- Endoribonucleases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametripartite motif containing 23
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Synonyms gene
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Synonyms gene name
- ADP-ribosylation factor domain protein 1, 64kDa
- tripartite motif-containing 23
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-06-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ARF GTPase family
- Ring finger proteins
- Tripartite motif containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameN-alpha-acetyltransferase 10, NatA catalytic subunit
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Synonyms gene
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Synonyms gene name
- ARD1 homolog, N-acetyltransferase (S. cerevisiae)
- ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
- N(alpha)-acetyltransferase 10, NatA catalytic subunit
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- N-alpha-acetyltransferase subunits
- GCN5 related N-acetyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data