NELF Antibody, FITC Conjugated
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Catalog numberbs-11893R-FITC
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PricePlease ask
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Size0.1ml
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Long nameNELF Polyclonal Antibody, FITC Conjugated
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CategoryConjugated Primary Antibodies
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ConjugationFITC
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Host OrganismRabbit (Oryctolagus cuniculus)
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Target AntigenNELF
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SpecificityThis is a highly specific antibody against NELF.
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ModificationUnmodified
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Modification siteNone
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ClonalityPolyclonal
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ClonePolyclonal antibody
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Concentration1ug per 1ul
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SourceKLH conjugated synthetic peptide derived from human NELF
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Tested applicationsIF(IHC-P)
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Recommended dilutionsIF(IHC-P)(1:50-200)
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CrossreactivityHuman, Mouse, Rat
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Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen backgroundNELF-A (negative elongation factor A), NELF-B, NELF-C, NELF-D and NELF-E are components of the NELF complex, which is a a structure that negatively regulates Pol II-dependent transcription elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission494nm/518nm
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SynonymsNasal embryonic LHRH factor; Nasal embryonic luteinizing hormone releasing hormone factor; Nasal embryonic luteinizing hormone-releasing hormone factor; Nelf; NELF_HUMAN.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolNSMF, NELFCD, NELFA, NELFB, NELFE
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Short nameAnti-NELF
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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IsotypeImmunoglobulin G (IgG)
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LabelFITC
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Alternative nameAnti-NELF PAb FITC
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameNMDA receptor synaptonuclear signaling and neuronal migration factor
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Synonyms gene
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Synonyms gene name
- nasal embryonic LHRH factor
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Locus
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Discovery year2004-03-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenegative elongation factor complex member C/D
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Synonyms gene
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Synonyms gene name
- TH1-like (Drosophila homolog)
- TH1-like (Drosophila)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
- Negative elongation factor complex members
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenegative elongation factor complex member A
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Synonyms gene
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Synonyms gene name
- Wolf-Hirschhorn syndrome candidate 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-07-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Negative elongation factor complex members
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenegative elongation factor complex member B
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Synonyms gene
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Synonyms gene name
- cofactor of BRCA1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-02-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Negative elongation factor complex members
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenegative elongation factor complex member E
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Synonyms gene
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Synonyms gene name
- RD RNA-binding protein
- RD RNA binding protein
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-11-18
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Entrez gene record
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Classification
- Negative elongation factor complex members
- MicroRNA protein coding host genes
- RNA binding motif containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data