NELF Antibody, Biotin Conjugated

  • Catalog number
    bs-11893R-Biotin
  • Price
    Please ask
  • Size
    0.1ml
  • Long name
    NELF Polyclonal Antibody, Biotin Conjugated
  • Category
    Conjugated Primary Antibodies
  • Conjugation
    Biotin
  • Host Organism
    Rabbit (Oryctolagus cuniculus)
  • Target Antigen
    NELF
  • Specificity
    This is a highly specific antibody against NELF.
  • Modification
    Unmodified
  • Modification site
    None
  • Clonality
    Polyclonal
  • Clone
    Polyclonal antibody
  • Concentration
    1ug per 1ul
  • Source
    KLH conjugated synthetic peptide derived from human NELF
  • Tested applications
    WB, IHC-P
  • Recommended dilutions
    WB(1:100-1000), IHC-P(1:100-500)
  • Crossreactivity
    Human, Mouse, Rat
  • Crossreactive species details
    Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
  • Antigen background
    NELF-A (negative elongation factor A), NELF-B, NELF-C, NELF-D and NELF-E are components of the NELF complex, which is a a structure that negatively regulates Pol II-dependent transcription elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT.
  • Purification method
    This antibody was purified via Protein A.
  • Storage conditions
    Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
  • Excitation Emission
    N/A
  • Synonyms
    Nasal embryonic LHRH factor; Nasal embryonic luteinizing hormone releasing hormone factor; Nasal embryonic luteinizing hormone-releasing hormone factor; Nelf; NELF_HUMAN.
  • Properties
    If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Additional conjugation
    Biotinylated
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    NSMF, NELFCD, NELFA, NELFB, NELFE
  • Short name
    Anti-NELF
  • Technique
    Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
  • Isotype
    Immunoglobulin G (IgG)
  • Label
    Biotin
  • Alternative name
    Anti-NELF PAb Biotin
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    NMDA receptor synaptonuclear signaling and neuronal migration factor
  • Synonyms gene
  • Synonyms gene name
    • nasal embryonic LHRH factor
  • Locus
  • Discovery year
    2004-03-25
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • MicroRNA protein coding host genes
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    negative elongation factor complex member A
  • Synonyms gene
  • Synonyms gene name
    • Wolf-Hirschhorn syndrome candidate 2
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-07-24
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Negative elongation factor complex members
    • MicroRNA protein coding host genes
  • VEGA ID
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    negative elongation factor complex member E
  • Synonyms gene
  • Synonyms gene name
    • RD RNA-binding protein
    • RD RNA binding protein
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2002-11-18
  • Entrez gene record
  • Classification
    • Negative elongation factor complex members
    • MicroRNA protein coding host genes
    • RNA binding motif containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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