NEDD8 Antibody
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Catalog numberF42040-0.4ML
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PricePlease ask
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenNEDD8
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100
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NotesTitration of the NEDD8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis NEDD8 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ15843
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PurityPurified
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DescriptionNEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
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ImmunogenA portion of amino acids 1-32 from the human protein was used as the immunogen for this NEDD8 antibody.
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StorageAliquot the NEDD8 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolNEDD8-MDP1, NEDD8
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Short nameAnti-NEDD8
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to NEDD8
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameNEDD8-MDP1 readthrough
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Locus
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Discovery year2011-02-21
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameNEDD8 ubiquitin like modifier
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Synonyms gene name
- neural precursor cell expressed, developmentally down-regulated 8
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-01-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data